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Effect of different culture media on isolation and differentiation of dendritic cells

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dc.contributor.authorYALÇIN, AHMET SUHA
dc.contributor.authorsYilmaz, Ayse Mine; Altundag, Ergul Mutlu; Gedik, Gulsah; Kocturk, Semra; Yalcin, A. Suha; Taga, Yavuz
dc.date.accessioned2022-03-13T12:50:35Z
dc.date.available2022-03-13T12:50:35Z
dc.date.issued2015
dc.description.abstractObjective: Dendritic cells (DCs) are members of the mammalian immune system and are considered to be the most powerful antigen presenting cells. They are responsible for the induction of T-cells or T-cell dependent immunity and tolerance. In this study we have investigated the effect of different serum supplements on generation and yield of mature dendritic cells isolated from peripheral blood mononuclear cells. Methods: Three different serum supplements (10% Fetal Bovine Serum, 1% Human Serum Albumin and 1% autologous serum) were compared with serum-free media to identify the role and importance of serum supplements on DC cultivation. Effect of different media on maturation signs (CD40, CD80, CD86, CD209a) and cytokine release (TNF-alpha, IL-10, IL-12, IL-6) was examined. Results: DCs generated in serum-free media was similar to those of cells in medium with autologous serum. Few dendritic-like cells were observed in fetal bovine serum and human serum albumin. The effect of different media on maturation of DCs was compared phenotypically and increased expression of CD80, CD86 and CD209a identified maturation and yield of DCs. Conclusion: Our results suggest that serum free media can be used to overcome potential drawbacks associated with different serum containing supplements.
dc.identifier.doi10.1515/tjb-2015-0031
dc.identifier.eissn1303-829X
dc.identifier.issn0250-4685
dc.identifier.urihttps://hdl.handle.net/11424/238388
dc.identifier.wosWOS:000368907400002
dc.language.isoeng
dc.publisherWALTER DE GRUYTER GMBH
dc.relation.ispartofTURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDendritic cells
dc.subjectperipheral blood mononuclear cells
dc.subjectserum-free media
dc.subjectCD209a
dc.subjectIL-10
dc.subjectIL-12
dc.subjectPERIPHERAL-BLOOD
dc.subjectLANGERHANS CELLS
dc.subjectIN-VITRO
dc.subjectHEMATOPOIETIC PROGENITORS
dc.subjectBONE-MARROW
dc.subjectT-CELLS
dc.subjectDC-SIGN
dc.subjectGENERATION
dc.subjectMACROPHAGES
dc.subjectTOLERANCE
dc.titleEffect of different culture media on isolation and differentiation of dendritic cells
dc.typearticle
dspace.entity.typePublication
local.avesis.id96c7a62f-5aee-4c15-8fc4-e53ebd7891f9
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atTRDIZIN
local.journal.numberofpages8
oaire.citation.endPage447
oaire.citation.issue6
oaire.citation.startPage440
oaire.citation.titleTURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
oaire.citation.volume40
relation.isAuthorOfPublication7a2bc583-aab6-43e7-9cc3-0d7a60773409
relation.isAuthorOfPublication.latestForDiscovery7a2bc583-aab6-43e7-9cc3-0d7a60773409

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