Publication:
Evaluation of biochemical parameters inRubus tereticaulistreated rats and its implications in wound healing

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dc.contributor.authorŞEN, ALİ
dc.contributor.authorsAksoy, Halil; Demirbag, Caglar; Sen, Ali; Sekerler, Turgut; Ozakpinar, Ozlem; Sener, Azize; Ahmad, Sarfraz; Tetik, Sermin
dc.date.accessioned2022-03-12T22:54:47Z
dc.date.available2022-03-12T22:54:47Z
dc.date.issued2020
dc.description.abstractWe evaluated the effects ofRubus tereticaulisin healing process by determining the total carbonyl content, collagen synthesis, and total protein level on rat wounded tissues. Wounds were performed in the back of 54 Wistar rats, using a biopsy punch instrument with 0.6 mm in diameter. Rats were randomly divided into three groups: (i) un-treatment wounds group served as controls, (ii) Madecassol (R) used as positive control group, and (iii) the application of topical cream ofR. tereticaulisserved as treatment group of wound healing. The animals were killed at the end of experiment under anesthesia with ketamine, and tissue samples were collected for the evaluation at three times intervals (3rd, 7th, and 14th day). The wounded areas were analyzed for total carbonyl content, collagen, and total protein levels by HPLC, ELISA, and spectrophotometric methods, respectively. Total carbonyl content in the treatment group was significantly lower in comparison with control group on 3rd day (2.839 +/- 0.438 vs. 3.216 +/- 0.216 nmol carbonyl/mol protein;p < 0.5) and 14th days (4.222 +/- 0.128 vs. 4.784 +/- 0.077 nmol carbonyl/mol protein;p < 0.05), respectively. New collagen formation on the wound sites after the initial injury was noted in the treated and positive control groups (5.310 +/- 0.331 vs. 5.164 +/- 0.377 mg collagen/g wet tissue) at the 3rd day than control group (2.180 +/- 0.718 mg collagen/g wet tissue,p < 0.01), and in treated and positive control groups at 7th day (9.654 +/- 0.201, 9.053 +/- 1.062 mg collagen/g wet tissue,p < 0.01); and in treated and positive control groups at 14th day (8.469 +/- 0.236, 5.631 +/- 0.531 mg collagen/g wet tissue, respectively;p < 0.05) in comparison with the control group. Total protein level of samples did not change significantly between the groups. Thus, application ofR. tereticaulisameliorated the wound healing process in rats as it facilitated collagen formation through healing of the wound. Evaluating total carbonyl content by HPLC could be useful as an advance procedure for quantification of healing.
dc.identifier.doi10.1007/s11010-020-03784-w
dc.identifier.eissn1573-4919
dc.identifier.issn0300-8177
dc.identifier.pubmed32524320
dc.identifier.urihttps://hdl.handle.net/11424/236521
dc.identifier.wosWOS:000539511400001
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofMOLECULAR AND CELLULAR BIOCHEMISTRY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectWound healing
dc.subjectRubus tereticaulis
dc.subjectcarbonyl content
dc.subjectHPLC
dc.subjectBiochemical assessments
dc.subjectRat
dc.subjectPROTEINS
dc.subjectPLASMA
dc.subjectDAMAGE
dc.titleEvaluation of biochemical parameters inRubus tereticaulistreated rats and its implications in wound healing
dc.typearticle
dspace.entity.typePublication
local.avesis.ida87405dc-5762-46bd-a3e2-9ec4d84f2af7
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages12
local.journal.quartileQ3
oaire.citation.endPage78
oaire.citation.issue1-2
oaire.citation.startPage67
oaire.citation.titleMOLECULAR AND CELLULAR BIOCHEMISTRY
oaire.citation.volume472
relation.isAuthorOfPublication9e088078-d757-41a4-afc6-909f32031cb1
relation.isAuthorOfPublication.latestForDiscovery9e088078-d757-41a4-afc6-909f32031cb1

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