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Proteomic response of Escherichia coli to the alkaloid extract of Papaver polychaetum

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsOzbalci, Cagakan; Unsal, Caglayan; Kazan, Dilek; Sariyar-Akbulut, Berna
dc.date.accessioned2022-03-12T17:48:27Z
dc.date.available2022-03-12T17:48:27Z
dc.date.issued2010
dc.description.abstractThe cellular response of Escherichia coli exposed to alkaloids extracted from a biennial endemic plant, Papaver polychaetum, was explored using proteome analysis. Following determination of the minimum inhibitory concentration of the berberine-containing plant extract as 1,250 mu g/mL, E. coli cells were grown in the presence of 750 mu g/mL extract. The response of the bacteria to the extract, with berberine found as the major alkaloid, was analyzed on two-dimensional gels. The differentially expressed proteins in the presence of 750 mu g/mL extract were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. These proteins included those that play vital roles for maintenance such as protein synthesis (elongation factor-Ts), transport (oligopeptide-binding protein A, uncharacterized amino-acid ABC transporter ATP binding protein YECC), energy metabolism (alpha-subunit of ATP synthase, pyridine nucleotide transhydrogenase STHA) and regulation. These results provide clues for understanding the mechanism of the alkaloid extract-induced stress and cytotoxicity on E. coli. The altered proteins can serve as potential targets for development of innovative therapeutic agents.
dc.identifier.doi10.1007/s13213-010-0118-0
dc.identifier.eissn1869-2044
dc.identifier.issn1590-4261
dc.identifier.urihttps://hdl.handle.net/11424/229958
dc.identifier.wosWOS:000285047700016
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofANNALS OF MICROBIOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectProteomics
dc.subjectAntimicrobial
dc.subjectEscherichia coli
dc.subjectPapaver polychaetum
dc.subjectBerberine
dc.subjectANTIMICROBIAL ACTIVITY
dc.subjectBINDING PROTEIN
dc.subjectBERBERINE
dc.subjectDNA
dc.subjectEXPRESSION
dc.subjectGENE
dc.subjectCLEAVAGE
dc.subjectGENOME
dc.subjectMODE
dc.titleProteomic response of Escherichia coli to the alkaloid extract of Papaver polychaetum
dc.typearticle
dspace.entity.typePublication
local.avesis.idc0b24d26-eaa5-45eb-88c4-a44c36238127
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages9
oaire.citation.endPage717
oaire.citation.issue4
oaire.citation.startPage709
oaire.citation.titleANNALS OF MICROBIOLOGY
oaire.citation.volume60
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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