Publication: alpha-Amylase immobilization on functionalized nano CaCO3 by covalent attachment
| dc.contributor.author | KAHRAMAN, MEMET VEZİR | |
| dc.contributor.authors | Demir, Serap; Gok, Sevda Burcu; Kahraman, Memet Vezir | |
| dc.date.accessioned | 2022-03-12T18:05:11Z | |
| dc.date.available | 2022-03-12T18:05:11Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | In this study, a-amylase was immobilized on glutaraldehyde activated silanized calcium carbonate nanoparticles by a using covalent binding method. The surface modified nano calcium carbonate (CaCO3) were characterized using FTIR and SEM. Immobilization yield was found as 199.43 mg/g of calcium carbonate nanoparticles. The maximum activity was observed at pH 6.5. The immobilized enzyme had a higher activity at elevated temperature (5090 degrees C) than the free one. Reuse studies demonstrated that the immobilized enzyme could reuse 25 times while retaining 18.2% of its activity. Free enzyme lost its activity completely within 15 days. Vmax values for the free and immobilized enzymes were calculated as 10 and 0.35 mg/mL/min, respectively. | |
| dc.identifier.doi | 10.1002/star.201100058 | |
| dc.identifier.eissn | 1521-379X | |
| dc.identifier.issn | 0038-9056 | |
| dc.identifier.uri | https://hdl.handle.net/11424/230627 | |
| dc.identifier.wos | WOS:000298744100001 | |
| dc.language.iso | eng | |
| dc.publisher | WILEY-V C H VERLAG GMBH | |
| dc.relation.ispartof | STARCH-STARKE | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | a-Amylase | |
| dc.subject | Covalent Immobilization | |
| dc.subject | Enzyme | |
| dc.subject | Glutaraldehyde | |
| dc.subject | Nano CaCO3 | |
| dc.subject | ENZYME | |
| dc.subject | PERFORMANCE | |
| dc.subject | STABILITY | |
| dc.subject | CHITOSAN | |
| dc.subject | SURFACE | |
| dc.subject | MATRIX | |
| dc.subject | TOOL | |
| dc.title | alpha-Amylase immobilization on functionalized nano CaCO3 by covalent attachment | |
| dc.type | article | |
| dspace.entity.type | Publication | |
| local.avesis.id | 1134cd3c-5f52-45a2-9e15-61f716d088f1 | |
| local.import.package | SS17 | |
| local.indexed.at | WOS | |
| local.indexed.at | SCOPUS | |
| local.journal.numberofpages | 7 | |
| oaire.citation.endPage | 9 | |
| oaire.citation.issue | 1 | |
| oaire.citation.startPage | 3 | |
| oaire.citation.title | STARCH-STARKE | |
| oaire.citation.volume | 64 | |
| relation.isAuthorOfPublication | 7676f3ad-0384-4001-b17d-b928ae2de7e6 | |
| relation.isAuthorOfPublication.latestForDiscovery | 7676f3ad-0384-4001-b17d-b928ae2de7e6 |