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Extremophilic Natrinema versiforme Against Pseudomonas aeruginosa Quorum Sensing and Biofilm

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsBasaran, Tunahan Irmak; Berber, Didem; Gokalsin, Baris; Tramice, Annabella; Tommonaro, Giuseppina; Abbamondi, Gennaro Roberto; Erginer Haskoylu, Merve; Toksoy oner, Ebru; Iodice, Carmine; Sesal, Nuzhet Cenk
dc.date.accessioned2022-03-14T10:53:30Z
dc.date.available2022-03-14T10:53:30Z
dc.date.issued2020-02-06
dc.description.abstractPseudomonas aeruginosa is an opportunistic pathogen that causes high morbidity and mortality rates due to its biofilm form. Biofilm formation is regulated via quorum sensing (QS) mechanism and provides up to 1000 times more resistance against conventional antibiotics. QS related genes are expressed according to bacterial population density via signal molecules. QS inhibitors (QSIs) from natural sources are widely studied evaluating various extracts from extreme environments. It is suggested that extremely halophilic Archaea may also produce QSI compounds. For this purpose, we tested QS inhibitory potentials of ethyl acetate extracts from cell free supernatants and cells of Natrinema versiforme against QS and biofilm formation of P. aeruginosa. To observe QS inhibition, all extracts were tested on P. aeruginosa lasB-gfp, rhlA-gfp, and pqsA-gfp biosensor strains and biofilm inhibition was studied using P. aeruginosa PAO1. According to our results, QS inhibition ratios of cell free supernatant extract (CFSE) were higher than cell extract (CE) on las system, whereas CE was more effective on rhl system. In addition, anti-biofilm effect of CFSE was higher than CE. Structural analysis revealed that the most abundant compound in the extracts was trans 4-(2-carboxy-vinyl) benzoic acid.
dc.identifier.doi10.3389/fmicb.2020.00079
dc.identifier.issn1664-302X
dc.identifier.pubmed32117114
dc.identifier.urihttps://hdl.handle.net/11424/245330
dc.identifier.wosWOS:000517217800001
dc.language.isoeng
dc.publisherFRONTIERS MEDIA SA
dc.relation.ispartofFRONTIERS IN MICROBIOLOGY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPseudomonas aeruginosa
dc.subjectextremophiles
dc.subjectquorum sensing
dc.subjectbiofilm
dc.subjectantivirulence
dc.subjectNatrinema versiforme
dc.subjectN-ACYLHOMOSERINE LACTONASE
dc.subjectTHERMOPHILIC BACTERIUM
dc.subjectHALOPHILIC ARCHAEON
dc.subjectVIRULENCE
dc.subjectIDENTIFICATION
dc.subjectSTRATEGIES
dc.subjectSYSTEMS
dc.subjectDRUGS
dc.subjectACID
dc.titleExtremophilic Natrinema versiforme Against Pseudomonas aeruginosa Quorum Sensing and Biofilm
dc.typearticle
dspace.entity.typePublication
local.avesis.id67dbae46-31d8-4540-8da9-ab5ddad6bca2
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumber79
local.journal.numberofpages10
local.journal.quartileQ1
oaire.citation.titleFRONTIERS IN MICROBIOLOGY
oaire.citation.volume11
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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