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The Role of Multilayer Electrospun Poly(Vinyl Alcohol)/Gelatin nanofibers loaded with Fluconazole and Cinnamaldehyde in the Potential Treatment of Fungal Keratitis

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dc.contributor.authorGÜNDÜZ, OĞUZHAN
dc.contributor.authorTINAZ, GÜLGÜN
dc.contributor.authorŞENGÖR, MUSTAFA
dc.contributor.authorCESUR, SÜMEYYE
dc.contributor.authorsIlhan E., Cesur S., Sulutas R. B., Pilavci E., Dalbayrak B., Kaya E., Arisan E. D., Tinaz G., ŞENGÖR M., Kijeenska-Gawaronska E., et al.
dc.date.accessioned2023-08-04T06:40:04Z
dc.date.available2023-08-04T06:40:04Z
dc.date.issued2022-08-01
dc.description.abstractFungal keratitis is a severe corneal infection that causes irreversible damage to the cornea, for which conventional drug treatments may be insufficient. With the new generation of drug delivery systems, it is desired to ensure the ocular usability of drugs. In this study, two-layer polyvinyl alcohol and gelatin (PVA/GEL) nanofibers with high drug loading capacity were produced by the electrospinning method. Cinnamaldehyde (CA), an FDAapproved volatile molecule found in cinnamon essential oil, and fluconazole (FLU), an antifungal drug, were incorporated into PVA/GEL nanofibers to inhibit the growth and biofilm formation of Candida albicans, one of the pathogens that cause fungal keratitis. The morphology, chemical structures, and thermal transitions of the produced pure (PVA/GEL), FLU loaded (PVA/GEL/FLU), CA loaded (PVA/GEL/CA), and combined FLU and CA loaded (PVA/GEL/COM) nanofibers were analysed by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC), respectively. The mechanical analysis, swelling and degradation behaviour, and drug release kinetics of the nanofibers were investigated. PVA/ GEL/FLU, PVA/GEL/CA and PVA/GEL/FLU/COM nanofibers were evaluated for their antifungal and antibiofilm activity against Candida albicans. Results showed that PVA/GEL/FLU and PVA/GEL/COM nanofibers have significant antifungal activity and inhibited biofilm formation by 37% and 49%, respectively. Furthermore, it was determined by MTT analysis using a human embryonic kidney (HEK) that the nanofibers were not cytotoxic. In the treatment of fungal keratitis, double-layer PVA/GEL/COM nanofiber with CA in the first layer and FLU in the second layer can create a new treatment approach as an alternative drug delivery system.
dc.identifier.citationIlhan E., Cesur S., Sulutas R. B., Pilavci E., Dalbayrak B., Kaya E., Arisan E. D., Tinaz G., ŞENGÖR M., Kijeenska-Gawaronska E., et al., "The Role of Multilayer Electrospun Poly(Vinyl Alcohol)/Gelatin nanofibers loaded with Fluconazole and Cinnamaldehyde in the Potential Treatment of Fungal Keratitis", EUROPEAN POLYMER JOURNAL, cilt.176, 2022
dc.identifier.doi10.1016/j.eurpolymj.2022.111390
dc.identifier.issn0014-3057
dc.identifier.urihttps://hdl.handle.net/11424/292068
dc.identifier.volume176
dc.language.isoeng
dc.relation.ispartofEUROPEAN POLYMER JOURNAL
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectKimya
dc.subjectFizikokimya
dc.subjectPolimer Karakterizasyonu
dc.subjectTemel Bilimler
dc.subjectChemistry
dc.subjectPhysical Chemistry
dc.subjectCharacterization of Polymers
dc.subjectNatural Sciences
dc.subjectPOLİMER BİLİMİ
dc.subjectTemel Bilimler (SCI)
dc.subjectPOLYMER SCIENCE
dc.subjectCHEMISTRY
dc.subjectNatural Sciences (SCI)
dc.subjectPolimerler ve Plastikler
dc.subjectKimya (çeşitli)
dc.subjectGenel Kimya
dc.subjectFizik Bilimleri
dc.subjectPolymers and Plastics
dc.subjectChemistry (miscellaneous)
dc.subjectGeneral Chemistry
dc.subjectPhysical Sciences
dc.subjectFungal keratitis
dc.subjectNanofiber
dc.subjectFluconazole
dc.subjectCinnamaldehyde
dc.subjectCornea tissue engineering
dc.subjectDIAGNOSIS
dc.subjectSCAFFOLDS
dc.subjectFIBERS
dc.subjectMATS
dc.subjectFungal keratitis
dc.subjectNanofiber
dc.subjectFluconazole
dc.subjectCinnamaldehyde
dc.subjectCornea tissue engineering
dc.titleThe Role of Multilayer Electrospun Poly(Vinyl Alcohol)/Gelatin nanofibers loaded with Fluconazole and Cinnamaldehyde in the Potential Treatment of Fungal Keratitis
dc.typearticle
dspace.entity.typePublication
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local.indexed.atWOS
local.indexed.atSCOPUS
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