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Omics of Selenium Biology: A Prospective Study of Plasma Proteome Network Before and After Selenized-Yeast Supplementation in Healthy Men

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dc.contributor.authorARĞA, KAZIM YALÇIN
dc.contributor.authorsSinha, Indu; Karagoz, Kubra; Fogle, Rachel L.; Hollenbeak, Christopher S.; Zea, Arnold H.; Arga, Kazim Y.; Stanley, Anne E.; Hawkes, Wayne C.; Sinha, Raghu
dc.date.accessioned2022-03-12T20:30:12Z
dc.date.available2022-03-12T20:30:12Z
dc.date.issued2016
dc.description.abstractLow selenium levels have been linked to a higher incidence of cancer and other diseases, including Keshan, Chagas, and Kashin-Beck, and insulin resistance. Additionally, muscle and cardiovascular disorders, immune dysfunction, cancer, neurological disorders, and endocrine function have been associated with mutations in genes encoding for selenoproteins. Selenium biology is complex, and a systems biology approach to study global metabolomics, genomics, and/or proteomics may provide important clues to examining selenium-responsive markers in circulation. In the current investigation, we applied a global proteomics approach on plasma samples collected from a previously conducted, double-blinded placebo controlled clinical study, where men were supplemented with selenized-yeast (Se-Yeast; 300g/day, 3.8mol/day) or placebo-yeast for 48 weeks. Proteomic analysis was performed by iTRAQ on 8 plasma samples from each arm at baseline and 48 weeks. A total of 161 plasma proteins were identified in both arms. Twenty-two proteins were significantly altered following Se-Yeast supplementation and thirteen proteins were significantly changed after placebo-yeast supplementation in healthy men. The differentially expressed proteins were involved in complement and coagulation pathways, immune functions, lipid metabolism, and insulin resistance. Reconstruction and analysis of protein-protein interaction network around selected proteins revealed several hub proteins. One of the interactions suggested by our analysis, PHLD-APOA4, which is involved in insulin resistance, was subsequently validated by Western blot analysis. Our systems approach illustrates a viable platform for investigating responsive proteomic profile in before and after' condition following Se-Yeast supplementation. The nature of proteins identified suggests that selenium may play an important role in complement and coagulation pathways, and insulin resistance.
dc.identifier.doi10.1089/omi.2015.0187
dc.identifier.eissn1557-8100
dc.identifier.issn1536-2310
dc.identifier.pubmed27027327
dc.identifier.urihttps://hdl.handle.net/11424/234158
dc.identifier.wosWOS:000374760800002
dc.language.isoeng
dc.publisherMARY ANN LIEBERT, INC
dc.relation.ispartofOMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectINTEGRATIVE ANALYSIS
dc.subjectINSULIN-RESISTANCE
dc.subjectPROSTATE-CANCER
dc.subjectBINDING PROTEIN
dc.subjectBREAST-CANCER
dc.subjectSERUM
dc.subjectAMERICAN
dc.subjectTRIAL
dc.subjectASSOCIATION
dc.subjectBIOMARKERS
dc.titleOmics of Selenium Biology: A Prospective Study of Plasma Proteome Network Before and After Selenized-Yeast Supplementation in Healthy Men
dc.typearticle
dspace.entity.typePublication
local.avesis.id89e08c54-7caf-427d-a7d7-29622f280f66
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages12
local.journal.quartileQ2
oaire.citation.endPage213
oaire.citation.issue4
oaire.citation.startPage202
oaire.citation.titleOMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
oaire.citation.volume20
relation.isAuthorOfPublication3582c2d4-7fbb-4db7-8e90-75613782b894
relation.isAuthorOfPublication.latestForDiscovery3582c2d4-7fbb-4db7-8e90-75613782b894

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