Publication:
Role of Ovarian Hormones in Psychological Stress-induced Oxidative Organ Damage in Rats

Simple item page
dc.contributor.authorYEGEN, BERRAK
dc.contributor.authorsMemi, Gulsun; Yegen, Berrak C.
dc.date.accessioned2022-03-12T20:28:27Z
dc.date.available2022-03-12T20:28:27Z
dc.date.issued2016
dc.description.abstractObjective: Stress response varies with respect to gender via the hypothalamic-pituitary-gonadal axis. We aimed to investigate the effect of ovarian hormone deficiency on psychological stress response and oxidative damage. Methods: Female Sprague Dawley rats (250-300 g, n=56) were divided as control, sham, and ovariectomy (OVX) groups. Sham operation or surgical OVX were conducted under anesthesia. After 60 days, the rats were placed in a special chamber to induce psychological stress by electric shock and were kept in the same chamber for 30 min on the following 3 days. Glucocorticoid receptor antagonist RU-486 (10 mg/kg), oxytocin receptor antagonist atosiban (1 mg/kg), or saline was intraperitoneally administered 10 min before stress exposure. After the hole-board anxiety test, the rats were decapitated on the 4th day; tissue and blood samples were obtained. Results: Psychological stress increased cortisol levels in the RU-486-administered group, while cortisol levels were decreased in the atosiban-administered group. Serum interleukin (IL)-1 ss levels, but not TNF-alpha levels, were increased by inducing stress. Stress increased oxidative damage in the stomach, colon, and brain of ovariectomized rats (p<0.05-0.001), while atosiban partially reversed and RU-486 exaggerated oxidative damage. GSH levels that were depleted because of stress were partially replenished by administering atosiban; however, RU-486 had no effect on GSH levels. Conclusion: Although the absence of ovarian hormones during psychological stress had no effect on cortisol or anxiety levels, changes in cytokine levels and oxidative tissue damage were observed.
dc.identifier.doi10.5152/clinexphealthsci.2016.04
dc.identifier.issn2459-1459
dc.identifier.urihttps://hdl.handle.net/11424/233918
dc.identifier.wosWOS:000435279300004
dc.language.isotur
dc.publisherAVES PRESS LTD
dc.relation.ispartofCLINICAL AND EXPERIMENTAL HEALTH SCIENCES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHypothalamopituitary-adrenal axis
dc.subjecthypothalamopituitary gonadal axis
dc.subjectglucocorticoid receptor
dc.subjectatosiban
dc.subjectoxytocin
dc.titleRole of Ovarian Hormones in Psychological Stress-induced Oxidative Organ Damage in Rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id8449139b-7487-4292-87d0-5545066d5232
local.import.packageSS17
local.indexed.atWOS
local.indexed.atTRDIZIN
local.journal.numberofpages8
oaire.citation.endPage79
oaire.citation.issue2
oaire.citation.startPage72
oaire.citation.titleCLINICAL AND EXPERIMENTAL HEALTH SCIENCES
oaire.citation.volume6
relation.isAuthorOfPublicatione4eaf9ac-f8dc-4e2b-b940-895cc906790d
relation.isAuthorOfPublication.latestForDiscoverye4eaf9ac-f8dc-4e2b-b940-895cc906790d

Files

Collections

Research Outputs