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Nesfatin-1 treatment preserves antioxidant status and attenuates renal fibrosis in rats with unilateral ureteral obstruction

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dc.contributor.authorÇETİNEL, ŞULE
dc.contributor.authorYEGEN, BERRAK
dc.contributor.authorKAYA, ÖZLEM TUĞÇE
dc.contributor.authorÖZBEYLİ, DİLEK
dc.contributor.authorsTezcan N., Özdemir-Kumral Z. N., Yenal N. Ö., Çilingir-Kaya Ö. T., Virlan A. T., Özbeyli D., Çetinel Ş., Yeğen B., Koç M.
dc.date.accessioned2023-03-02T07:44:51Z
dc.date.available2023-03-02T07:44:51Z
dc.date.issued2022-06-01
dc.description.abstractBackground Nesfatin-1 (NES-1), an anorexigenic peptide, was reported to have anti-inflammatory and anti-apoptotic actions in several inflammation models. Methods To elucidate potential renoprotective effects of NES-1, unilateral ureteral obstruction (UUO) was induced in male Sprague Dawley rats by ligating left ureters. The rats were injected intraperitoneally with either saline (SL) or NES-1 (10 mu g/kg/day) for 7 or 14 days (n = 8 in each group). On the 7th or 14th day, obstructed kidneys were removed for the isolation of leucocytes for flow-cytometric analysis and the assessments of biochemical and histopathological changes. Results Opposite to glutathione levels, renal myeloperoxidase activity in the SL-treated UUO group was significantly increased compared with the sham-operated group, while NES-1 treatment abolished the elevation. The percentages of CD8+/CD4+ T-lymphocytes infiltrating the obstructed kidneys were increased in the SL-treated groups but treatment with NES-1 did not prevent lymphocyte infiltration. Elevated tumour necrosis factor-alpha (TNF-alpha) levels in SL-treated UUO group were decreased with NES-1. Although total degeneration scores were similarly increased in all UUO groups, tubular dilatation scores were significantly increased in UUO groups and lowered by NES-1 only in the 7-day treated group. Elevated interstitial fibrosis scores in the SL-treated groups were decreased in both 7- and 14-day NES-1 treated groups, while alpha-smooth muscle actin (alpha-SMA) and apoptosis scores were depressed in both NES-1 treated groups. Conclusion The present data demonstrate that UUO-induced renal fibrosis is ameliorated by NES-1, which appears to involve the inhibition of neutrophil infiltration and thereby amelioration of oxidative stress and inflammation. These data suggest that NES-1 may have a regulatory role in protecting the kidneys against obstruction-induced renal injury.
dc.identifier.citationTezcan N., Özdemir-Kumral Z. N., Yenal N. Ö., Çilingir-Kaya Ö. T., Virlan A. T., Özbeyli D., Çetinel Ş., Yeğen B., Koç M., "Nesfatin-1 treatment preserves antioxidant status and attenuates renal fibrosis in rats with unilateral ureteral obstruction", NEPHROLOGY DIALYSIS TRANSPLANTATION, cilt.37, sa.7, ss.1238-1248, 2022
dc.identifier.doi10.1093/ndt/gfac053
dc.identifier.endpage1248
dc.identifier.issn0931-0509
dc.identifier.issue7
dc.identifier.startpage1238
dc.identifier.urihttps://hdl.handle.net/11424/287069
dc.identifier.volume37
dc.language.isoeng
dc.relation.ispartofNEPHROLOGY DIALYSIS TRANSPLANTATION
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectTıp
dc.subjectDahili Tıp Bilimleri
dc.subjectİç Hastalıkları
dc.subjectNefroloji
dc.subjectSağlık Bilimleri
dc.subjectMedicine
dc.subjectInternal Medicine Sciences
dc.subjectInternal Diseases
dc.subjectNephrology
dc.subjectHealth Sciences
dc.subjectTRANSPLANTASYON
dc.subjectKlinik Tıp
dc.subjectKlinik Tıp (MED)
dc.subjectÜROLOJİ VE NEFROLOJİ
dc.subjectTRANSPLANTATION
dc.subjectCLINICAL MEDICINE
dc.subjectClinical Medicine (MED)
dc.subjectUROLOGY & NEPHROLOGY
dc.subjectÜroloji
dc.subjectTransplantasyon
dc.subjectUrology
dc.subjectTransplantation
dc.subjectapoptosis
dc.subjectinflammation
dc.subjectnesfatin-1
dc.subjectoxidative stress
dc.subjectunilateral ureteral obstruction
dc.subjectBLOOD-BRAIN-BARRIER
dc.subjectPROGRESSIVE FIBROSIS
dc.subjectEXPRESSION
dc.subjectINJURY
dc.subjectNEPHROPATHY
dc.subjectDAMAGE
dc.subjectANTAGONIST
dc.subjectAPOPTOSIS
dc.subjectBLOCKADE
dc.titleNesfatin-1 treatment preserves antioxidant status and attenuates renal fibrosis in rats with unilateral ureteral obstruction
dc.typearticle
dspace.entity.typePublication
local.avesis.id701ce290-4304-4ac7-81c3-7f4ed66d5657
local.indexed.atWOS
local.indexed.atPUBMED
local.indexed.atSCOPUS
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relation.isAuthorOfPublication.latestForDiscoverycd39227a-5dec-4ac2-818b-36ef87a2d15c

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