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Analysis of quorum sensing-dependent virulence factor production and its relationship with antimicrobial susceptibility in Pseudomonas aeruginosa respiratory isolates

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dc.contributor.authorKARAHASAN, AYŞEGÜL
dc.contributor.authorsKaratuna, O.; Yagci, A.
dc.date.accessioned2022-03-14T10:04:26Z
dc.date.available2022-03-14T10:04:26Z
dc.date.issued2010-12
dc.description.abstractP>Pseudomonas aeruginosa is an opportunistic pathogen causing severe respiratory infections. The pathogenesis of these infections is multifactorial and the production of many virulence factors is regulated by quorum sensing (QS), a cell-to-cell communication mechanism. The two well defined QS systems in P. aeruginosa, the las and rhl systems, rely on N-acyl homoserine lactone signal molecules, also termed autoinducers. We assessed the activity of QS-dependent virulence factors (including elastase, alkaline protease, pyocyanin and biofilm production) in respiratory isolates of P. aeruginosa and their relationship with antimicrobial susceptibility. We identified sixteen isolates displaying impaired phenotypic activity; among them, eleven isolates were also defective in autoinducer production, and therefore considered QS-deficient. Six of the QS-deficient isolates failed to amplify one or more of the four QS regulatory genes (lasI, lasR, rhlI, rhlR) with PCR: one isolate was negative for rhlR, two isolates were negative for rhlI and rhlR and three isolates were negative for all four genes. The isolates that were negative for virulence factor production were generally less susceptible to the antimicrobials and statistically significant correlations were observed between the lack of elastase production and resistance to piperacillin and ceftazidime; between failure in alkaline protease production and resistance to tobramycin, piperacillin, piperacillin-tazobactam, cefepime, imipenem and ciprofloxacin; and between failure in pyocyanin production and resistance to amikacin, tobramycin, ceftazidime, ciprofloxacin and ofloxacin. The results obtained indicate that, despite the pivotal role of QS in the pathogenesis of P. aeruginosa respiratory infections, QS-deficient strains are still capable of causing infections and tend to be less susceptible to antimicrobials.
dc.identifier.doi10.1111/j.1469-0691.2010.03177.x
dc.identifier.eissn1469-0691
dc.identifier.issn1198-743X
dc.identifier.pubmed20132256
dc.identifier.urihttps://hdl.handle.net/11424/244004
dc.identifier.wosWOS:000284170400013
dc.language.isoeng
dc.publisherELSEVIER SCI LTD
dc.relation.ispartofCLINICAL MICROBIOLOGY AND INFECTION
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntimicrobial susceptibility
dc.subjectPseudomonas aeruginosa
dc.subjectquorum sensing
dc.subjectvirulence
dc.subjectMULTIDRUG EFFLUX SYSTEM
dc.subjectTO-CELL SIGNALS
dc.subjectFACTOR EXPRESSION
dc.subjectCYSTIC-FIBROSIS
dc.subjectELASTASE
dc.subjectGENES
dc.titleAnalysis of quorum sensing-dependent virulence factor production and its relationship with antimicrobial susceptibility in Pseudomonas aeruginosa respiratory isolates
dc.typearticle
dspace.entity.typePublication
local.avesis.id6dcf3709-3f64-4942-8064-2732fafd016e
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage1775
oaire.citation.issue12
oaire.citation.startPage1770
oaire.citation.titleCLINICAL MICROBIOLOGY AND INFECTION
oaire.citation.volume16
relation.isAuthorOfPublicationd8b6c313-4dc1-4253-adf4-6e2e6d68af90
relation.isAuthorOfPublication.latestForDiscoveryd8b6c313-4dc1-4253-adf4-6e2e6d68af90

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