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Bioassay-guided isolation and antiproliferative efficacy of extract loaded in chitosan nanoparticles and LC-QTOF-MS/MS analysis of Achillea magnifica

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dc.contributor.authorTAŞKIN, TURGUT
dc.contributor.authorsTaskin, Turgut; Dogan, Murat; Arabaci, Turan
dc.date.accessioned2022-03-12T22:43:42Z
dc.date.available2022-03-12T22:43:42Z
dc.date.issued2020
dc.description.abstractThis study investigated the antioxidant, anti-urease, anticholinesterase, and antiproliferative activities of different extracts of A. magnifica. The active compounds were isolated from chloroform extract, which has the strongest antiproliferative and antioxidant activities. The structures of the isolated compounds (elenolic acid, luteolin, eupatilin) were examined using spectroscopic techniques (NMR, UV, HR-MS). The antioxidant and antiproliferative effects of isolated compounds showed lower biological activity than those of the chloroform extract. Therefore, chitosan nanoparticles were prepared with this extract and characterized. The cytotoxic activities of the chitosan nanoparticles were examined, and they were found to have a strong antiproliferative activity similar to that of the chloroform extract. The ethanol extract had strong anti-urease and anticholinesterase activity compared with other extracts. Therefore, phenolic compounds that might be responsible for the biological activity were analysed by LC-QTOF-MS/MS. The ferulic acid derivative, apigenin 6 C pentoside 8 C hexoside, apigenin 6,8-di C-hexoside, vitexin, and diosmetin 8-C-glucoside (orientin 4'-methyl ether) compounds from this plant were analysed for the first time. The results showed that the chloroform-loaded chitosan nanoparticles had a strong antiproliferative activity in breast cancer cell lines (MCF-7, MDA-MB-231) and no toxic effect on normal cell lines (L-929), which suggested that this extract might be a potential candidate as a herbal medicine that can be investigated and evaluated in future cancer studies. Additionally, the potential use of ethanol extract as an anti-urease and anticholinesterase agent was revealed. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.sajb.2020.08.002
dc.identifier.eissn1727-9321
dc.identifier.issn0254-6299
dc.identifier.urihttps://hdl.handle.net/11424/236352
dc.identifier.wosWOS:000571185800011
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofSOUTH AFRICAN JOURNAL OF BOTANY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectA. magnifica
dc.subjectChitosan nanoparticles
dc.subjectBiological activity
dc.subjectLC-QTOF-MS/MS
dc.subjectANTIOXIDANT ACTIVITY
dc.subjectESSENTIAL OILS
dc.subjectFLAVONOIDS
dc.subjectSYSTEM
dc.titleBioassay-guided isolation and antiproliferative efficacy of extract loaded in chitosan nanoparticles and LC-QTOF-MS/MS analysis of Achillea magnifica
dc.typearticle
dspace.entity.typePublication
local.avesis.id7afb25c0-fe53-49f0-8c81-eeacc7cf3322
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages9
local.journal.quartileQ2
oaire.citation.endPage244
oaire.citation.startPage236
oaire.citation.titleSOUTH AFRICAN JOURNAL OF BOTANY
oaire.citation.volume133
relation.isAuthorOfPublication265414e9-485c-42b8-903a-c467f6b49b84
relation.isAuthorOfPublication.latestForDiscovery265414e9-485c-42b8-903a-c467f6b49b84

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