Publication:
Specified Species in Gingival Crevicular Fluid Predict Bacterial Diversity

dc.contributor.authorDOĞAN, BAŞAK
dc.contributor.authorsAsikainen, Sirkka; Dogan, Basak; Turgut, Zeynep; Paster, Bruce J.; Bodur, Aysen; Oscarsson, Jan
dc.date.accessioned2022-03-14T09:05:34Z
dc.date.available2022-03-14T09:05:34Z
dc.date.issued2010-10-25
dc.description.abstractBackground: Analysis of gingival crevicular fluid (GCF) samples may give information of unattached (planktonic) subgingival bacteria. Our study represents the first one targeting the identity of bacteria in GCF. Methodology/Principal Findings: We determined bacterial species diversity in GCF samples of a group of periodontitis patients and delineated contributing bacterial and host-associated factors. Subgingival paper point (PP) samples from the same sites were taken for comparison. After DNA extraction, 16S rRNA genes were PCR amplified and DNA-DNA hybridization was performed using a microarray for over 300 bacterial species or groups. Altogether 133 species from 41 genera and 8 phyla were detected with 9 to 62 and 18 to 64 species in GCF and PP samples, respectively, per patient. Projection to latent structures by means of partial least squares (PLS) was applied to the multivariate data analysis. PLS regression analysis showed that species of genera including Campylobacter, Selenomonas, Porphyromonas, Catonella, Tannerella, Dialister, Peptostreptococcus, Streptococcus and Eubacterium had significant positive correlations and the number of teeth with low-grade attachment loss a significant negative correlation to species diversity in GCF samples. OPLS/O2PLS discriminant analysis revealed significant positive correlations to GCF sample group membership for species of genera Campylobacter, Leptotrichia, Prevotella, Dialister, Tannerella, Haemophilus, Fusobacterium, Eubacterium, and Actinomyces. Conclusions/Significance: Among a variety of detected species those traditionally classified as Gram-negative anaerobes growing in mature subgingival biofilms were the main predictors for species diversity in GCF samples as well as responsible for distinguishing GCF samples from PP samples. GCF bacteria may provide new prospects for studying dynamic properties of subgingival biofilms.
dc.identifier.doi10.1371/journal.pone.0013589
dc.identifier.issn1932-6203
dc.identifier.pubmed21049043
dc.identifier.urihttps://hdl.handle.net/11424/242459
dc.identifier.wosWOS:000283422100009
dc.language.isoeng
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.ispartofPLOS ONE
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPERIODONTAL-DISEASE
dc.subjectMICROFLORA
dc.subjectIDENTIFICATION
dc.subjectINFLAMMATION
dc.subjectCOMPONENTS
dc.subjectMARKERS
dc.subjectCARIES
dc.titleSpecified Species in Gingival Crevicular Fluid Predict Bacterial Diversity
dc.typearticle
dspace.entity.typePublication
local.avesis.id90a3eb78-fa3f-4510-855c-87a9d1781354
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.articlenumbere13589
local.journal.numberofpages12
oaire.citation.issue10
oaire.citation.titlePLOS ONE
oaire.citation.volume5
relation.isAuthorOfPublicationa2569f78-7a2a-4f98-8cc9-d8b67ae56f6d
relation.isAuthorOfPublication.latestForDiscoverya2569f78-7a2a-4f98-8cc9-d8b67ae56f6d

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