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Water Miscible Mono Alcohols' Effect on the Proteolytic Performance of Bacillus clausii Serine Alkaline Protease

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dc.contributor.authorKAZAN, DİLEK
dc.contributor.authorsDuman, Yonca (Avci); Kazan, Dilek; Denizci, Aziz Akin; Erarslan, Altan
dc.date.accessioned2022-03-13T12:45:59Z
dc.date.available2022-03-13T12:45:59Z
dc.date.issued2014
dc.description.abstractIn this study, our investigations showed that the increasing concentrations of all examined mono alcohols caused a decrease in the V (m), k (cat) and k (cat)/K (m) values of Bacillus clausii GMBE 42 serine alkaline protease for casein hydrolysis. However, the K (m) value of the enzyme remained almost the same, which was an indicator of non-competitive inhibition. Whereas inhibition by methanol was partial non-competitive, inhibition by the rest of the alcohols tested was simple non-competitive. The inhibition constants (K (I)) were in the range of 1.32-3.10 M, and the order of the inhibitory effect was 1-propanol > 2-propanol > methanol > ethanol. The Delta G (not equal) and Delta G (not equal) (E -aEuro parts per thousand T) values of the enzyme increased at increasing concentrations of all alcohols examined, but the Delta G (not equal) (ES) value of the enzyme remained almost the same. The constant K (m) and Delta G (not equal) (ES) values in the presence and absence of mono alcohols indicated the existence of different binding sites for mono alcohols and casein on enzyme the molecule. The k (cat) of the enzyme decreased linearly by increasing log P and decreasing dielectric constant (D) values, but the Delta G (not equal) and Delta G (not equal) (E -aEuro parts per thousand T) values of the enzyme increased by increasing log P and decreasing D values of the reaction medium containing mono alcohols.
dc.identifier.doi10.1007/s12010-013-0525-3
dc.identifier.eissn1559-0291
dc.identifier.issn0273-2289
dc.identifier.pubmed24092453
dc.identifier.urihttps://hdl.handle.net/11424/237876
dc.identifier.wosWOS:000331005200039
dc.language.isoeng
dc.publisherHUMANA PRESS INC
dc.relation.ispartofAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBacillus clausii
dc.subjectAlkaline protease
dc.subjectMono alcohols
dc.subjectProteolytic performance
dc.subjectORGANIC-SOLVENTS
dc.subjectDIELECTRIC-CONSTANT
dc.subjectCATALYTIC-ACTIVITY
dc.subjectPENICILLIN V
dc.subjectSTABILITY
dc.subjectENZYMES
dc.subjectBIOCATALYSIS
dc.subjectTHERMOLYSIN
dc.subjectMIXTURES
dc.subjectDENATURATION
dc.titleWater Miscible Mono Alcohols' Effect on the Proteolytic Performance of Bacillus clausii Serine Alkaline Protease
dc.typearticle
dspace.entity.typePublication
local.avesis.ide1e622e0-c634-4619-a288-980d985f3d4c
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages18
oaire.citation.endPage486
oaire.citation.issue1
oaire.citation.startPage469
oaire.citation.titleAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
oaire.citation.volume172
relation.isAuthorOfPublication3972a0fe-adf3-4da2-b289-7f01f80e3689
relation.isAuthorOfPublication.latestForDiscovery3972a0fe-adf3-4da2-b289-7f01f80e3689

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