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Distinct functional muscarinic receptors in guinea-pig gallbladder, ileum and atria

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dc.contributor.authorAKICI, AHMET
dc.contributor.authorsKaraalp, A; Akici, A; Akbulut, H; Ulusoy, NB; Oktay, S
dc.date.accessioned2022-03-12T16:59:08Z
dc.date.available2022-03-12T16:59:08Z
dc.date.issued1999
dc.description.abstractObjective. The contractile responses of guinea-pig gallbladder smooth muscle cells have been suggested to be mediated by M-3 and M-4 muscarinic receptors by different research groups. Therefore, in the present study, several pharmacological properties of cholinergic functions in guinea-pig gallbladder, guinea-pig ileum (mediated via M-3 receptors), and guinea-pig and rat atria (mediated via Mt receptors) were compared, Methods: The isometric contractions of isolated guinea-pig ileum, guinea-pig gallbladder, guinea-pig and rat atrial strips in in vitro organ bath were recorded on a polygraph and the effects of carbachol, oxotremorine, McN-A-343, and clozapine have been investigated. Results: Three muscarinic receptor agonists, carbachol, oxotremorine and McN-A-343 showed different order of potencies in their negative inotropic effects and contractile actions in guinea-pig gallbladder suggesting that functional muscarinic receptors in the gallbladder are distinct from those in the atria, and similar to M-4-subtypes. Clozapine which was shown to have antagonistic affinity for muscarinic M-1, M-2, M-3 and M-5, but partial agonistic affinity for muscarinic M-4 receptors, contracted gallbladder concentration-dependently. On the other hand, clozapine antagonised carbachol-induced ileal and gallbladder contractions and negative inotropic effects indicating that it acts like a partial agonist in the gallbladder. Conclusion: It was concluded that the contractile muscarinic receptors of guinea-pig gallbladder are distinct from those of atria (M-2) and ileum (M-3), but seem to be of M-4 subtype. (C) 1999 Academic Press.
dc.identifier.doi10.1006/phrs.1999.0453
dc.identifier.issn1043-6618
dc.identifier.pubmed10328997
dc.identifier.urihttps://hdl.handle.net/11424/227155
dc.identifier.wosWOS:000080798600009
dc.language.isoeng
dc.publisherACADEMIC PRESS LTD
dc.relation.ispartofPHARMACOLOGICAL RESEARCH
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectmuscarinic
dc.subjectguinea-pig
dc.subjectgallbladder
dc.subjectileum
dc.subjectatria
dc.subjectMcN-A 343
dc.subjectoxotremorine
dc.subjectcarbachol
dc.subjectclozapine
dc.subjectSMOOTH-MUSCLE
dc.subjectM-4 RECEPTORS
dc.subjectCHO-K1 CELLS
dc.subjectSUBTYPES
dc.subjectBINDING
dc.subjectHETEROGENEITY
dc.subjectANTAGONIST
dc.subjectCLOZAPINE
dc.subjectCONTRACTION
dc.subjectCYCLASE
dc.titleDistinct functional muscarinic receptors in guinea-pig gallbladder, ileum and atria
dc.typearticle
dspace.entity.typePublication
local.avesis.ida31cfe54-9aa2-4904-a66c-e3a67173516b
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
oaire.citation.endPage395
oaire.citation.issue5
oaire.citation.startPage389
oaire.citation.titlePHARMACOLOGICAL RESEARCH
oaire.citation.volume39
relation.isAuthorOfPublication6c7c9f26-63a9-4f6f-91cd-aa9e3452919c
relation.isAuthorOfPublication.latestForDiscovery6c7c9f26-63a9-4f6f-91cd-aa9e3452919c

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