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Response Assessment With Molecular Characterization of Circulating Tumor Cells and Plasma MicroRNA Profiling in Patients With Locally Advanced Breast Cancer During Neoadjuvant Chemotherapy

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dc.contributor.authorERZİK, CAN
dc.contributor.authorsAkkiprik, Mustafa; Koca, Sinan; Ugurlu, M. Umit; Ekren, Ruchan; Eyuboglu, Irem Peker; Alan, Ozkan; Erzik, Can; Amuran, Gokce Gullu; Telli, Tugba Akin; Gulluoglu, M. Bahadir; Sezerman, Ugur; Yumuk, Perran Fulden
dc.date.accessioned2022-03-12T22:43:43Z
dc.date.available2022-03-12T22:43:43Z
dc.date.issued2020
dc.description.abstractPeripheral blood samples from 36 patients with locally advanced breast cancer who had undergone neoadjuvant chemotherapy were collected for circulating tumor cell (CTC) and plasma microRNA (miR) analysis. Pretreatment CTC and ALDH1 positivity (P = .0245) correlated, with miR-146b-5p and miR-199a-5p accompanied by CTC positivity. CTC and miR profiling of serial samples during neoadjuvant chemotherapy appears to be a very useful in predicting cure and clinical course. Background: Cells detaching from the primary tumor site are metastasis initiator cells, and the detection of CTC, known as liquid biopsy, is an important test of biomarkers of cancer progression. We investigated the molecular characterization of circulating tumor cells (CTCs), profiled the plasma microRNA (miR) content, and analyzed the relationship with the clinical outcomes by sampling the peripheral blood from patients with locally advanced breast cancer before and after neoadjuvant chemotherapy. Patients and Methods: Markers of breast cancer, epithelial-mesenchymal transition (EMT), drug resistance, and stem cells were used for CTC isolation and characterization. Plasma miR profiles were obtained from selected patients with CTC positivity determined using next-generation sequencing. Resutts: The proportion of CTC, EMT, and stem cell marker positivity was 16.7%, 8.3%, and 25% before and 18.2%, 15.2%, and 9.1% after treatment, respectively. A significant correlation was found between the pretreatment CTCs and ALDH1 positivity (P= .0245). These CTCs with stemness properties were observed in most hormone receptor-positive, human epidermal growth factor receptor 2 -negative cases and were also present with a high incidence in cases of early metastasis. miR-146b-5p and miR-199a-5p, which are involved in metastasis, invasion, and EMT, were accompanied by CTC positivity, and miR-4646-3p was associated with the development of early metastasis. Conclusions: Molecular characterization of CTCs and miR profiling of serial samples from patients with locally advanced breast cancer during neoadjuvant chemotherapy appears to be a very useful in predicting cure and clinical course and might be a key to developing new targeted therapies. (C) 2020 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.clbc.2020.02.006
dc.identifier.eissn1938-0666
dc.identifier.issn1526-8209
dc.identifier.pubmed32201164
dc.identifier.urihttps://hdl.handle.net/11424/236355
dc.identifier.wosWOS:000558628300023
dc.language.isoeng
dc.publisherCIG MEDIA GROUP, LP
dc.relation.ispartofCLINICAL BREAST CANCER
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBreast cancer
dc.subjectCTCs
dc.subjectEMT
dc.subjectNACT
dc.subjectStem cell marker
dc.subjectDIFFERENTIAL EXPRESSION ANALYSIS
dc.subjectRNA
dc.subjectBIOMARKER
dc.subjectMIRNAS
dc.subjectDIAGNOSIS
dc.titleResponse Assessment With Molecular Characterization of Circulating Tumor Cells and Plasma MicroRNA Profiling in Patients With Locally Advanced Breast Cancer During Neoadjuvant Chemotherapy
dc.typearticle
dspace.entity.typePublication
local.avesis.ide5039722-94a6-4941-93a9-018ccde4e8a4
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages15
local.journal.quartileQ3
oaire.citation.endPage+
oaire.citation.issue4
oaire.citation.startPage332
oaire.citation.titleCLINICAL BREAST CANCER
oaire.citation.volume20
relation.isAuthorOfPublication5081a883-9590-4d5d-8e2a-f83c8916241d
relation.isAuthorOfPublication.latestForDiscovery5081a883-9590-4d5d-8e2a-f83c8916241d

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