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Urinary micro-RNA expressions and protein concentrations may differentiate bladder cancer patients from healthy controls

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dc.contributor.authorILGIN, CAN
dc.contributor.authorsAmuran, Gokce Gullu; Tinay, Ilker; Filinte, Deniz; Ilgin, Can; Eyuboglu, Irem Peker; Akkiprik, Mustafa
dc.date.accessioned2022-03-12T22:28:12Z
dc.date.available2022-03-12T22:28:12Z
dc.date.issued2020
dc.description.abstractPurpose To determine expression differences of urine exosomal miR-19b1-5p, 21-5p, 136-3p, 139-5p, 210-3p and concentration differences of urinary BLCA-4, NMP22, APE1/Ref1, CRK, VIM between bladder cancer, follow-up patients, and control samples, to evaluate diagnostic importance of these differences and establish a diagnostic panel for bladder cancer. Methods Urine samples of 59 bladder cancer patients, 34 healthy controls, and 12 follow-up patients without recurrence were enrolled to this study. Real-time PCR and ELISA were performed to determine urine exosomal miR-19b1-5p, 21-5p, 136-3p, 139-5p, 210-3p expressions and urinary BLCA-4, NMP22, APE1/Ref1, CRK, VIM, creatinine concentrations. Logistic regression analyses were performed to determine the diagnostic panel, the sensitivity, and specificity of the panel assessed by the ROC curve analysis. p values < 0.05 were considered statistically significant. Results In bladder cancer risk groups, mir-139, -136, -19 and 210 expressions or positivity were found to be different and concentrations of urinary Ape1/Ref1, BLCA4, CRK, and VIM increased by twofold on average compared to healthy controls. Logistic regression and ROC analyses revealed that panel could differentiate bladder cancer patients from healthy controls with 80% sensitivity and 88% specificity (AUC = 0.899), low-risk patients from controls with 93% sensitivity and 95.5% specificity (AUC = 0.976). Despite the low number of samples, our findings suggest that urine exosomal miR-19b1-5p, 136-3p, 139-5p expression, and urinary APE1/Ref1, BLCA-4, CRK concentrations are promising candidates in terms of bladder cancer diagnosis. Conclusions Although our panel has great sensitivity for early detection of BC, it needs to be validated in larger populations.
dc.identifier.doi10.1007/s11255-019-02328-6
dc.identifier.eissn1573-2584
dc.identifier.issn0301-1623
dc.identifier.pubmed31679136
dc.identifier.urihttps://hdl.handle.net/11424/235294
dc.identifier.wosWOS:000493669000001
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofINTERNATIONAL UROLOGY AND NEPHROLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBladder cancer
dc.subjectUrinary protein
dc.subjectUrine exosomal miRNA
dc.subjectUrinary biomarker
dc.subjectBladder cancer diagnosis
dc.subjectBIOMARKERS
dc.subjectNORMALIZATION
dc.subjectDIAGNOSIS
dc.subjectSURVEILLANCE
dc.titleUrinary micro-RNA expressions and protein concentrations may differentiate bladder cancer patients from healthy controls
dc.typearticle
dspace.entity.typePublication
local.avesis.id3a084f04-890d-4586-905f-c534f4f9b29a
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages8
local.journal.quartileQ3
oaire.citation.endPage468
oaire.citation.issue3
oaire.citation.startPage461
oaire.citation.titleINTERNATIONAL UROLOGY AND NEPHROLOGY
oaire.citation.volume52
relation.isAuthorOfPublication380f62e4-4745-4e22-b9c9-67cb0373648d
relation.isAuthorOfPublication.latestForDiscovery380f62e4-4745-4e22-b9c9-67cb0373648d

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