Publication:
alpha-lipoic acid protects against renal ischaemia-reperfusion injury in rats

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dc.contributor.authorYÜKSEL, MERAL
dc.contributor.authorsSehirli, Oezer; Sener, Emre; Cetinel, Ule; Yueksel, Meral; Gedik, Nursal; Sener, Goeksel
dc.date.accessioned2022-03-12T17:34:05Z
dc.date.available2022-03-12T17:34:05Z
dc.date.issued2008
dc.description.abstract1. Oxygen free radicals are important components involved in the pathophysiological processes observed during ischaemia-reperfusion (I/R). The present study was designed to assess the possible protective effect of et-lipoic acid (ALA) on renal I/R injury. 2. Wistar albino rats were unilaterally nephrectomized and subjected to 45 min renal pedicle occlusion followed by 24 h reperfusion. Saline or ALA (100 mg/kg, i.p.) was administered 15 min prior to ischaemia and immediately before the reperfusion period. At the end of 24 h, rats were decapitated and trunk blood was collected. Creatinine, blood urea nitrogen (BUN) and lactate dehydrogenase (LDH) activity were measured in serum samples, whereas tumour necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL-6, 8-hydroxydeoxyguanosine (8-OHdG) and total anti-oxidant capacity (AOC) were assayed in plasma samples. 3. Kidney samples were taken for the determination of tissue malondialdehyde (MDA) and glutathione (GSH) levels, as well as Na/K-ATPase and myeloperoxidase (MPO) activity. The formation of reactive oxygen species in renal tissue samples was monitored using a chemiluminescence (CL) technique with luminol and lucigenin probes. Oxidant-induced tissue fibrosis was determined by tissue collagen content and the extent of tissue injury was analysed microscopically. 4. Ischaemia-reperfusion caused a significant increases in blood creatinine, BUN, LDH, IL-1 beta, IL-6, TNF-alpha and 8-OHdG, whereas AOC was decreased. In kidney samples from the I/R group, MDA, MPO, collagen and CL levels were found to be increased significantly; however, glutathione levels and Na/KATPase activity were decreased. Conversely, ALA treatment reversed all these biochemical indices, as well as histopathological alterations induced by I/R. 5. In conclusion, these data suggest that ALA reverses I/R-induced oxidant responses and improves microscopic damage and renal function. Thus, it seems likely that ALA protects kidney tissues by inhibiting neutrophil infiltration, balancing the oxidant-anti-oxidant status and regulating the generation of inflammatory mediators.
dc.identifier.doi10.1111/j.1440-1681.2007.04810.x
dc.identifier.issn0305-1870
dc.identifier.pubmed17941895
dc.identifier.urihttps://hdl.handle.net/11424/228966
dc.identifier.wosWOS:000254296500003
dc.language.isoeng
dc.publisherBLACKWELL PUBLISHING
dc.relation.ispartofCLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcytokines
dc.subjectischaemia-reperfusion
dc.subjectkidney
dc.subjectalpha-lipoic acid
dc.subjectmyeloperoxidase
dc.subjectISCHEMIA/REPERFUSION INJURY
dc.subjectSUPEROXIDE-DISMUTASE
dc.subjectOXIDATIVE STRESS
dc.subjectREACTIVE OXYGEN
dc.subjectFREE-RADICALS
dc.subjectAGED RATS
dc.subjectFAILURE
dc.subjectANTIOXIDANT
dc.subjectMYELOPEROXIDASE
dc.subjectINFLAMMATION
dc.titlealpha-lipoic acid protects against renal ischaemia-reperfusion injury in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.idf9094d84-ab2e-4f0d-94af-359778aa82ac
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
oaire.citation.endPage255
oaire.citation.issue3
oaire.citation.startPage249
oaire.citation.titleCLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
oaire.citation.volume35
relation.isAuthorOfPublication8b13d479-2f3b-4180-bc71-7ad5a5625f1b
relation.isAuthorOfPublication.latestForDiscovery8b13d479-2f3b-4180-bc71-7ad5a5625f1b

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