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Alterations in the kinetic activity of aromatlc-L-amino acid decarboxylase and preliminary 2-DE investigation of the brains in a 6-OHDA induced Parkinson's disease rat model

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dc.contributor.authorOGAN, AYŞE
dc.contributor.authorONAT, FİLİZ
dc.contributor.authorGÜLHAN, REZZAN
dc.contributor.authorsGünel A., OGAN A., ONAT F., GÜLHAN R.
dc.date.accessioned2023-06-01T07:09:10Z
dc.date.available2023-06-01T07:09:10Z
dc.date.issued2003-07-01
dc.description.abstractObjective: The aim of this study was to isolate and purify the aromatic-L-amino acid decarboxylase (AADC,EC 4.1.1.28) enzyme rats from Parkinson\"s Disease (PD) induced and the healthy control group rat brains and compare the alterations in the kinetic activities of the isolated enzyme. The protein spots displaying on the 2-DE patterns of the diseased and the healthy control group crude rat brain homogenates were evaluated. Medhods: In this study, the Parkinson\"s Disease model was induced by injecting 6-hydroxydopamine into the brains of the rats. The PD model formation was successful in two rats out of three. Results: The AADC decarboxylase was isolated and partially purified by DEAE-Sephacel ion exchange chromatography from the brains of PD induced and healthy control animals to compare the kinetic activity of the enzyme. The kinetic activity of the enzyme was reduced 70% in the PD group compared to controls. In order to determine and correlate the alterations with PD, and the distribution of the proteins displayed by the crude brain homogenates of the diseased and the healthy control group both were investigated. Polyacrylamide gel electrophoresis (PAGE) of the crude brain homogenates under the native and denaturizing conditions displayed matching bands for both of the groups, while two dimensional electrophoresis (2-DE) patterns of the crude brain homogenates of the diseased and the control group displayed considerable differences. Conclusion: The results of this study confirm the power of 2-DE-PAGE technique of the proteome analysis. Currently only the proteome analysis enables the identification of disease correlated proteins.
dc.identifier.citationGünel A., OGAN A., ONAT F., GÜLHAN R., "Alterations in the kinetic activity of aromatlc-L-amino acid decarboxylase and preliminary 2-DE investigation of the brains in a 6-OHDA induced Parkinson's disease rat model", Marmara Medical Journal, cilt.16, sa.3, ss.179-187, 2003
dc.identifier.endpage187
dc.identifier.issn1019-1941
dc.identifier.issue3
dc.identifier.startpage179
dc.identifier.urihttps://dergipark.org.tr/tr/download/article-file/252863
dc.identifier.urihttps://hdl.handle.net/11424/289829
dc.identifier.volume16
dc.language.isoeng
dc.relation.ispartofMarmara Medical Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectMedicine
dc.subjectHealth Sciences
dc.subjectFundamental Medical Sciences
dc.subjectKlinik Tıp (MED)
dc.subjectKlinik Tıp
dc.subjectTIP, GENEL & DAHİLİ
dc.subjectClinical Medicine (MED)
dc.subjectCLINICAL MEDICINE
dc.subjectMEDICINE, GENERAL & INTERNAL
dc.subjectGenel Tıp
dc.subjectGeneral Medicine
dc.subject2-DE
dc.subjectAADC
dc.subjectParkinson Disease
dc.subjectProteome
dc.subjectProteome
dc.subjectParkinson Disease
dc.subjectAADC
dc.subject2-DE
dc.titleAlterations in the kinetic activity of aromatlc-L-amino acid decarboxylase and preliminary 2-DE investigation of the brains in a 6-OHDA induced Parkinson's disease rat model
dc.typearticle
dspace.entity.typePublication
local.avesis.id276f4228-a908-420a-a0d6-95762ae2e9ab
local.indexed.atSCOPUS
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relation.isAuthorOfPublication.latestForDiscovery58db3332-a5f8-4224-a021-aeea795f52fa

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