Publication:
Chemical profiles and biological potential of tuber extracts from Cyclamen coum Mill

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dc.contributor.authorŞENKARDEŞ, İSMAİL
dc.contributor.authorsMahomoodally, Mohamad Fawzi; Picot-Allain, Marie Carene Nancy; Zengin, Gokhan; Llorent-Martinez, Eulogio J.; Stefanucci, Azzurra; Gunes, Ak; Senkardes, Ismail; Tomczyk, Michal; Mollica, Adriano
dc.date.accessioned2022-03-12T22:58:49Z
dc.date.available2022-03-12T22:58:49Z
dc.date.issued2021
dc.description.abstractCyclamen coum Mill. used in traditional medicine has been reported to exhibit interesting biological activities. In this study, the ability of the C. coum tuber extracts to inhibit clinical enzymes, namely, cholinesterases, ty-rosinase, alpha-amylase, and alpha-glucosidase, as well as the antioxidant properties were assessed. High -performance liquid chromatography/electrospray ionization quadrupole-time -of-flight mass spectrometer (HPLC- ESI-Q -TOF-MS) was employed to obtain detailed phytochemical proiles and in silico studies were performed against the key clinical enzymes. HPLC- ESI-Q -TOF -MS results conirmed the presence of saponins cyclacoumin and cy-claminorin, which were previously identiied from C. coum tuber extracts. In addition, other phenolic com-pounds, namely, phloretin C -dihexoside, quercetin 3 -galactoside, and catechin, were also identiied from C. coum tuber extracts. The methanol extract showed interesting inhibition against tyrosinase (6 5. 87 mg kojic acid equivalent/g extract) and alpha-glucosidase (3 4. 41 mg acarbose equivalent / g extract). Low inhibition was observed against cholinesterases and alpha-amylase. The methanol also showed active antioxidant properties (expressed as Trolox equivalent [TE]), namely, radical scavenging and reducing potential (1 5. 17 and 21 .8 6 mg TE / g extract, for 2, 2 '- azino- bis(3-ethylbenzthiazoline -6 -sulfonic acid) and cupric reducing antioxidant capacity assays, re-spectively). In order to highlight the possible bioactive compounds responsible of the biological effect, phloretin C -diglucoside, catechin and quercetin 3 -galactoside were docked via in silico molecular modelling ap-proaches. For tyrosinase, quercetin 3 -galactoside showed better docking and free binding energy which was found to bind very strongly to the enzymatic cavity of tyrosinase (via hydrogen bonds to Glu322, His85, His263, Gly281 and pi-pi stacks to His263 and His259). Findings from this study tend to advocate for further in-vestigations focusing on the isolation of bioactive compounds from the methanol extract of C. coum tuber ex-tracts and assessment of their cytotoxicity and their bioactivity.
dc.identifier.doi10.1016/j.bcab.2021.102008
dc.identifier.eissn1878-8181
dc.identifier.urihttps://hdl.handle.net/11424/237238
dc.identifier.wosWOS:000663074800012
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofBIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCyclamen coum
dc.subjectMolecular docking
dc.subjectCholinesterases
dc.subjectAmylase
dc.subjectCyclacoumin
dc.subjectIN-VITRO
dc.subjectANTIOXIDANTS
dc.subjectPERSPECTIVE
dc.subjectSILICO
dc.subjectPARTS
dc.subjectPLANT
dc.titleChemical profiles and biological potential of tuber extracts from Cyclamen coum Mill
dc.typearticle
dspace.entity.typePublication
local.avesis.id0763b20e-5ff0-408e-ba47-f0618dcb94c9
local.import.packageSS17
local.indexed.atWOS
local.journal.articlenumber102008
local.journal.numberofpages7
oaire.citation.titleBIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
oaire.citation.volume33
relation.isAuthorOfPublication3e59035c-d7b1-4887-b78c-2a9bc207de05
relation.isAuthorOfPublication.latestForDiscovery3e59035c-d7b1-4887-b78c-2a9bc207de05

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