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Exploring the role of miRNAs in the diagnosis of MODY3

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dc.contributor.authorGÜLLÜ AMURAN, GÖKÇE
dc.contributor.authorsBaltaci, Oguzhan Fatih; Colakoglu, Seyma; Gullu Amuran, Gokce; Aydin, Neslihan; Sargin, Mehmet; Karabay, Arzu; Yilmaz, Temel; Berber, Ergul
dc.date.accessioned2022-04-25T00:11:23Z
dc.date.available2022-04-25T00:11:23Z
dc.date.issued2018
dc.description.abstractBackground/aim: MODY3 associated with HNF1A is the most common form of MODY and is clinically misdiagnosed as type 1 diabetes due to similar clinical symptoms. This study aimed to analyze the role of HNF1A-regulated miRNAs as a biomarker in the diagnosis of MODY3. Materials and methods: MIN6 cells were transfected with the HNF1A cDNA expression vector for overexpression or with siRNA specific to HNF1A to silence its expression. The HNF1A-regulated miRNAs were determined by RNA-Seq of the total RNA extract. Expressions of the candidate miRNAs in blood samples of MODY3, type 1 diabetes, and type 2 diabetes patients and in healthy subjects were compared statistically by Mann-Whitney U tests. Results: This study revealed the presence of 238 known HNF1A-regulated miRNAs in MIN6 cells. miR-129-1-3p, miR-200b-3p, and miR-378a-5p were selected as candidate miRNAs. The expression level of miR-378a-5p significantly decreased in type 2 diabetes and MODY3 patients, while miR-200b-3p expression was significantly decreased only in MODY3 patients. Conclusion: Although further studies with larger numbers of patients are required, this study demonstrated that the expression levels of miR-200b-3p and miR-378a-5p decrease in MODY3 patients and suggests that miR-200b-3p is an especially strong candidate to use clinically for selecting suspected MODY3 patients.
dc.identifier.doi10.3906/sag-1711-98
dc.identifier.eissn1303-6165
dc.identifier.issn1300-0144
dc.identifier.pubmed29916222
dc.identifier.urihttps://hdl.handle.net/11424/263893
dc.identifier.wosWOS:000435653900025
dc.languageeng
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
dc.relation.ispartofTURKISH JOURNAL OF MEDICAL SCIENCES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiomarker
dc.subjectdiabetes
dc.subjectmiRNA
dc.subjectMODY3
dc.subjectmiR-200
dc.subjectmiR-378
dc.subjectFACTOR-I
dc.subjectEXPRESSION
dc.subjectYOUNG
dc.subjectMUTATIONS
dc.subjectPROTEIN
dc.subjectMICRORNAS
dc.subjectGLUCOSE
dc.subjectGROWTH
dc.subjectHNF1A
dc.subjectALPHA
dc.titleExploring the role of miRNAs in the diagnosis of MODY3
dc.typearticle
dspace.entity.typePublication
local.avesis.id1ec7becc-ff2a-4e49-aed9-126a3764eb11
local.import.packageSS39
local.indexed.atWOS
local.journal.numberofpages13
local.journal.quartileQ4
oaire.citation.endPage+
oaire.citation.issue3
oaire.citation.startPage620
oaire.citation.titleTURKISH JOURNAL OF MEDICAL SCIENCES
oaire.citation.volume48
relation.isAuthorOfPublication6b40e64d-7dc3-4fdc-9d2d-7cf7f2f5c14c
relation.isAuthorOfPublication.latestForDiscovery6b40e64d-7dc3-4fdc-9d2d-7cf7f2f5c14c

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