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Exosomes' profile in ankylosing spondylitis: A preliminary study

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dc.contributor.authorABACAR, KEREM YİĞİT
dc.contributor.authorATAGÜNDÜZ, MEHMET PAMİR
dc.contributor.authorERZİK, CAN
dc.contributor.authorsKarakaya E., Deniz R., ABACAR K. Y., ATAGÜNDÜZ M. P., ERZİK C.
dc.date.accessioned2023-04-24T11:21:29Z
dc.date.available2023-04-24T11:21:29Z
dc.date.issued2023-01-01
dc.description.abstractObjective: Ankylosing spondylitis (AS) is a chronic systemic inflammatory disease that leads to structural and functional im-pairments and reduced quality of life, with heterogeneous manifestations. The origin and possible role of extracellular vesicles represented by exosomes (EVexo) in the pathogenesis of AS were examined in this study. Materials and Methods: Extracellular vesicles (EVs) were isolated from serum from ten AS patients and ten healthy controls through Izon qEV2/35 nm columns. After assessing the isolate purity by bicinchoninic acid assay (BCA) and Enzyme-Linked ImmunoSorbent Assay (ELISA), the relationship between EVexo concentration and AS was tested by the BCA method. The EVexo surface markers were analyzed by flow cytometry (FC) to verify EVexo presence and reveal its origin. Results: In FC analysis, CD86+TSG101+ and CD3+TSG101+ exosome percentages of AS group were significantly higher than the control group (p<0.05). A significant difference was found between the AS and control groups in terms of CD3+IL17+ and CD3+IFNg+ and CD86+TNF alpha+ and CD86+IL12(p35)+ exosome percentages (p<0.01). Conclusion: The exosomes whose ratio increased in the AS process were derived from T cells expressing increased levels of IL-17A and IFNg in their membranes, and macrophages expressing increased levels of TNF alpha and IL-12(p35) in their membranes. The EVexo profile did not change according to the AS course.
dc.identifier.citationKarakaya E., Deniz R., ABACAR K. Y., ATAGÜNDÜZ M. P., ERZİK C., "Exosomes' Profile in Ankylosing Spondylitis: A Preliminary Study", ERCIYES MEDICAL JOURNAL, cilt.45, sa.1, ss.32-40, 2023
dc.identifier.doi10.14744/etd.2022.54614
dc.identifier.endpage40
dc.identifier.issn2149-2247
dc.identifier.issue1
dc.identifier.startpage32
dc.identifier.urihttps://avesis.marmara.edu.tr/api/publication/8e2ab6fb-f9ca-4548-a2da-9af9b510a4aa/file
dc.identifier.urihttps://hdl.handle.net/11424/288896
dc.identifier.volume45
dc.language.isoeng
dc.relation.ispartofERCIYES MEDICAL JOURNAL
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectMedicine
dc.subjectHealth Sciences
dc.subjectFundamental Medical Sciences
dc.subjectTIP, GENEL & DAHİLİ
dc.subjectKlinik Tıp
dc.subjectKlinik Tıp (MED)
dc.subjectMEDICINE, GENERAL & INTERNAL
dc.subjectCLINICAL MEDICINE
dc.subjectClinical Medicine (MED)
dc.subjectGenel Sağlık Meslekleri
dc.subjectPatofizyoloji
dc.subjectTemel Bilgi ve Beceriler
dc.subjectDeğerlendirme ve Teşhis
dc.subjectDahiliye
dc.subjectAile Sağlığı
dc.subjectTıp (çeşitli)
dc.subjectGenel Tıp
dc.subjectGeneral Health Professions
dc.subjectPathophysiology
dc.subjectFundamentals and Skills
dc.subjectAssessment and Diagnosis
dc.subjectInternal Medicine
dc.subjectFamily Practice
dc.subjectMedicine (miscellaneous)
dc.subjectGeneral Medicine
dc.subjectExosome
dc.subjectextracellular vesicle
dc.subjectankylosing spondylitis
dc.subjectcell surface markers
dc.titleExosomes' profile in ankylosing spondylitis: A preliminary study
dc.typearticle
dspace.entity.typePublication
local.avesis.id8e2ab6fb-f9ca-4548-a2da-9af9b510a4aa
local.indexed.atWOS
relation.isAuthorOfPublication55769942-746f-4108-9df0-b79d2d29b3bc
relation.isAuthorOfPublication324d1d0f-ecfe-4b31-b5b9-1884a093b669
relation.isAuthorOfPublication5081a883-9590-4d5d-8e2a-f83c8916241d
relation.isAuthorOfPublication.latestForDiscovery55769942-746f-4108-9df0-b79d2d29b3bc

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