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Prognostic evidence of LEF1 isoforms in childhood acute lymphoblastic leukemia

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dc.contributor.authorTRUE, ÖMER
dc.contributor.authorsErbilgin, Yucel; Ng, Ozden Hatirnaz; Can, Ismail; Firtina, Sinem; Kucukcankurt, Fulya; Karaman, Serap; Karakas, Zeynep; Celkan, Tulin Tiraje; Zengin, Emine; Gelen, Sema Aylan; Ozdemir, Gul Nihal; Yildirmak, Yildiz; Dogru, Omer; Tansel, Turkan; Khodzhaev, Khusan; Toluk, Ozlem; Ozbek, Ugur; Sayitoglu, Muge
dc.date.accessioned2022-03-12T22:59:04Z
dc.date.available2022-03-12T22:59:04Z
dc.date.issued2021
dc.description.abstractIntroduction The lymphoid enhancer factor 1 (LEF1) is a DNA-binding transcription factor that functions in the Wnt signaling pathway. Increased LEF1 activity is associated with progression of several types of cancer including leukemia. Here, we investigated LEF1 isoform expression and genomic variations in acute lymphoblastic leukemia (ALL). Methods LEF1 isoform expression was evaluated by quantitative real-time PCR in 87 newly diagnosed childhood ALL patients and controls. Moreover, Western blot analysis was performed for detection of LEF1 expression and the hotspot region of LEF1 was screened by deep sequencing. Results The LEF1 mRNA expression of B cell ALL patients was higher than the controls (LEF1-total P = .011, LEF1-long P = .026). Moreover, B-ALL samples showing higher total LEF1 expression had significantly shorter relapse-free survival (P = .008) and overall survival (P = .011). Although full-length LEF1 expression was similar to the controls in T-ALL, 50% (n = 15) of the ALL patients had increased full-length LEF1 protein expression. Imbalance between short- and full-length LEF1 isoforms may lead to cell survival in ALL. Beside the LEF1 activation, LEF1 gene variations were rarely observed in our cohort. Conclusion The results indicate that the Wnt pathway may have a pathogenic function in a group of ALL patients and high LEF1-total expression might be a marker for shorter relapse-free survival time in B cell ALL.
dc.identifier.doi10.1111/ijlh.13513
dc.identifier.eissn1751-553X
dc.identifier.issn1751-5521
dc.identifier.pubmed33844466
dc.identifier.urihttps://hdl.handle.net/11424/237267
dc.identifier.wosWOS:000639287900001
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofINTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectALL
dc.subjectbiomarker
dc.subjectgene expression
dc.subjectLEF1
dc.subjectprognosis
dc.titlePrognostic evidence of LEF1 isoforms in childhood acute lymphoblastic leukemia
dc.typearticle
dspace.entity.typePublication
local.avesis.id8ac8a02a-d0c7-4d83-abc1-42bcdc7d4b63
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages11
oaire.citation.endPage1103
oaire.citation.issue5
oaire.citation.startPage1093
oaire.citation.titleINTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
oaire.citation.volume43
relation.isAuthorOfPublicatione3d558cd-4ea7-4175-a297-aedd91d8b47a
relation.isAuthorOfPublication.latestForDiscoverye3d558cd-4ea7-4175-a297-aedd91d8b47a

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