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The behavioral and neurochemical effects of methylprednisolone or metyrapone in a post-traumatic stress disorder rat model

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dc.contributor.authorAYDIN OMAY, BANU
dc.contributor.authorsTanriverdi, Ayse Melek; Aydin, Banu; Bebitoglu, Berna Terzioglu; Cabadak, Hulya; Goren, M. Zafer
dc.date.accessioned2022-03-14T09:16:54Z
dc.date.available2022-03-14T09:16:54Z
dc.date.issued2019
dc.description.abstractOBJECTIVE: Mechanisms contributing to the post-traumatic stress disorder (PTSD) that involve several physiological sys- tems, and the activation of the hypothalamic-pituitary-adrenal axis (HPA) is one of the most known systems in the PTSD pathophysiology. The present study investigates the potential effects of methylprednisolone, metyrapone and their association with the noradrenergic system within the rostral pons, a region containing the locus coeruleus (LC) in a rat model of PTSD induced with predator scent. METHODS: In this study, Sprague-Dawley rats were exposed to the stress by exposure to the scent of dirty cat litter, which is a natural stressor of a predator. One week later, the rats were re-exposed to a situational reminder (clean cat litter). The rats were treated using either methylprednisolone, metyrapone or physiological saline before exposure to a situational reminder (n=8 in each group). Noradrenaline (NA) levels in the rostral pons homogenates were analysed using ELISA. RESULTS: The anxiety indices of the rats exposed to the trauma were found to be significantly higher than the anxiety indices of the control rats. Metyrapone produced a significant increase in the anxiety indices of the non-stressed rats, and methylprednisolone did not produce a change in the anxiety indices of the non-stressed rats. Methylprednisolone treatment suppressed the anxiety in the stressed rats. Metyrapone treatment increased the anxiety indices in the stressed rats but still being lower than that of the saline-treated stressed rats. Significant decrease in the freezing time was observed following the methylprednisolone treatment both in the stressed and non-stressed rats. NA content in the rostral pons of the stressed rats was significantly higher than that of the non-stressed rats. Methylprednisolone or metyrapone treatments decreased the NA content in the non-stressed rats as compared to the saline treatment. However, these decreases were not significant. CONCLUSION: In this study, findings suggest that stress may give rise to endocrine, autonomic and behavioural responses. The anxiety indices and NA levels in the rostral pons increased with the traumatic event. The methylprednisolone treatment may suppress anxiety through interactions between the LC and the HPA axis.
dc.identifier.doi10.14744/nci.2019.69345
dc.identifier.issn2148-4902
dc.identifier.pubmed31909376
dc.identifier.urihttps://hdl.handle.net/11424/242900
dc.identifier.wosWOS:000500484500002
dc.language.isoeng
dc.publisherKARE PUBL
dc.relation.ispartofNORTHERN CLINICS OF ISTANBUL
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnxiety index
dc.subjecthypothalamic-pituitary-adrenal axis
dc.subjectlocus coeruleus
dc.subjectnoradrenaline
dc.subjectrostral pons
dc.subjectCORTICOTROPIN-RELEASING-FACTOR
dc.subjectLOCUS-COERULEUS
dc.subjectMEMORY
dc.subjectGLUCOCORTICOIDS
dc.subjectCORTISOL
dc.subjectFEAR
dc.subjectHYDROCORTISONE
dc.subjectDEXAMETHASONE
dc.subjectHIPPOCAMPUS
dc.subjectSYMPTOMS
dc.titleThe behavioral and neurochemical effects of methylprednisolone or metyrapone in a post-traumatic stress disorder rat model
dc.typearticle
dspace.entity.typePublication
local.avesis.id855be693-a80a-4d9c-821a-56049197c025
local.import.packageSS16
local.indexed.atWOS
local.indexed.atPUBMED
local.indexed.atTRDIZIN
local.journal.numberofpages7
oaire.citation.endPage333
oaire.citation.issue4
oaire.citation.startPage327
oaire.citation.titleNORTHERN CLINICS OF ISTANBUL
oaire.citation.volume6
relation.isAuthorOfPublicationaf9e48cc-cef6-4af2-bdb7-5b424aadfc2b
relation.isAuthorOfPublication.latestForDiscoveryaf9e48cc-cef6-4af2-bdb7-5b424aadfc2b

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