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Preparation and characterization of sol-gel hybrid coating films for covalent immobilization of lipase enzyme

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dc.contributor.authorOGAN, AYŞE
dc.contributor.authorsYuce-Dursun, Basak; Cigil, Asli Beyler; Dongez, Dilek; Kahraman, M. Vezir; Ogan, Ayse; Demir, Serap
dc.date.accessioned2022-03-12T20:27:36Z
dc.date.available2022-03-12T20:27:36Z
dc.date.issued2016
dc.description.abstractIn this study UV-curable hybrid epoxy-silica polymer films were prepared via sol-gel method. Lipase (EC 3.1.1.3) from Candida rugosa was covalently immobilized onto hybrid epoxy-silica polymer films and immobilization capacity of polymer films was found 7.22 mg g(-1). The morphology of the polymeric support was characterized by scanning electron microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Immobilized and free enzymes were used in two different reaction systems: hydrolysis of p-nitrophenyl palmitate in aqueous medium and synthesis of p-nitrophenyl linoleate (from p-nitrophenol and linoleic acid) in n-hexane medium. The effect of temperature on hydrolytic and synthetic activities was investigated and observed maximum activities at 50 degrees C and 45 degrees C for immobilized enzyme, orderly. Km values for free enzyme were determined 0.71 and 1.12 mM by hydrolytic and synthetic activity assays, respectively, while these values were observed as 0.91 mM and 1.19 mM for immobilized enzyme. At the end of 30 repeated cycles, 56% and 59% of initial activities remained for hydrolytic and synthetic assays, respectively. Native enzyme lost its activity completely within 20 days, whereas the immobilized enzyme retained for hydrolytic and synthetic activities was approximately 82% and 72%, respectively, under the same storage time. (C) 2016 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.molcatb.2016.02.007
dc.identifier.eissn1873-3158
dc.identifier.issn1381-1177
dc.identifier.urihttps://hdl.handle.net/11424/233730
dc.identifier.wosWOS:000374615000003
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectLipase
dc.subjectImmobilization
dc.subjectSol-gel
dc.subjectHydrolytic activity
dc.subjectSynthetic activity
dc.subjectCANDIDA-RUGOSA LIPASE
dc.subjectNANOFIBROUS MEMBRANES
dc.subjectADSORPTION
dc.subjectCHITOSAN
dc.subjectSILICA
dc.subjectAMYLASE
dc.subjectBEADS
dc.titlePreparation and characterization of sol-gel hybrid coating films for covalent immobilization of lipase enzyme
dc.typearticle
dspace.entity.typePublication
local.avesis.idc9a6af42-76ac-4950-94b2-410d42daaa35
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages8
local.journal.quartileQ3
oaire.citation.endPage25
oaire.citation.startPage18
oaire.citation.titleJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
oaire.citation.volume127
relation.isAuthorOfPublication58db3332-a5f8-4224-a021-aeea795f52fa
relation.isAuthorOfPublication.latestForDiscovery58db3332-a5f8-4224-a021-aeea795f52fa

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