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Melatonin supports alendronate in preserving bone matrix and prevents gastric inflammation in ovariectomized rats

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dc.contributor.authorYEGEN, BERRAK
dc.contributor.authorsGurler, Esra Bihter; Cilingir-Kaya, Ozlem Tugce; Eyuboglu, Irem Peker; Ercan, Feriha; Akkiprik, Mustafa; Reiter, Russell J.; Yegen, Berrak C.
dc.date.accessioned2022-03-12T22:38:43Z
dc.date.available2022-03-12T22:38:43Z
dc.date.issued2019
dc.description.abstractThe anti-catabolic bisphosphonate alendronate is considered as the first-line medical treatment in post-menopausal osteoporosis; but several side effects, including gastric mucosal injury, are associated with its use. The aim was to elucidate whether combined treatment with melatonin plus alendronate would be more advantageous in the maintenance of bone and the prevention of gastric side effects. Under anaesthesia, female Sprague-Dawley rats underwent bilateral ovariectomy (OVX), while control group had sham surgery. Four weeks after the surgery, OVX rats were treated with saline, melatonin (25 mu g/mL/d), alendronate (70 mu g/kg/wk), melatonin + alendronate, melatonin + melatonin receptor antagonist (luzindole, 10 mu g/kg/d) or alendronate + melatonin + luzindole for 8 weeks. Rats were euthanized at the end of 12th week. Runx2 expression, apoptotic cells, and trabecular thickness were evaluated in tibiae, while gastric tissues were analysed for oxidative injury parameters. In all OVX groups, Runx2 expression was depressed. Saline-treated OVX group presented an extreme decrease in calcified area in opposition to melatonin- or alendronate-treated groups, while the bones in alendronate + melatonin-treated group were similar to those of the sham-operated group. Concomitant with the improvements examined histologically in bone tissues, quantitative TUNEL (+) cells were similarly lower in alendronate- or melatonin-treated groups. Oxidative gastric damage was increased in saline- or alendronate-treated groups, which were depressed in the presence of melatonin. Although melatonin and alendronate exerted similar supportive effects on the maintenance of bone mass, melatonin may have a more advantageous impact by protecting against OVX-induced gastric injury, which was aggravated by alendronate use. Highlights Our results demonstrate that alendronate and melatonin had similar supportive effects on the maintenance of bone mass, while melatonin prevented the gastric side effects of alendronate, making this combination an advisable therapeutic approach in the treatment of menopausal osteoporosis.
dc.identifier.doi10.1002/cbf.3379
dc.identifier.eissn1099-0844
dc.identifier.issn0263-6484
dc.identifier.pubmed30815905
dc.identifier.urihttps://hdl.handle.net/11424/235714
dc.identifier.wosWOS:000461879900006
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofCELL BIOCHEMISTRY AND FUNCTION
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalendronate
dc.subjectgastric injury
dc.subjectmelatonin
dc.subjectosteoporosis
dc.subjectpost-menopausal
dc.subjectMESENCHYMAL STEM-CELLS
dc.subjectINJURY
dc.subjectDIFFERENTIATION
dc.subjectEXPRESSION
dc.titleMelatonin supports alendronate in preserving bone matrix and prevents gastric inflammation in ovariectomized rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id0747cb6f-9739-4803-8d4c-85cd28c0585c
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages11
local.journal.quartileQ3
oaire.citation.endPage112
oaire.citation.issue2
oaire.citation.startPage102
oaire.citation.titleCELL BIOCHEMISTRY AND FUNCTION
oaire.citation.volume37
relation.isAuthorOfPublicatione4eaf9ac-f8dc-4e2b-b940-895cc906790d
relation.isAuthorOfPublication.latestForDiscoverye4eaf9ac-f8dc-4e2b-b940-895cc906790d

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