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Basophil activation test for inhalant allergens in pediatric patients with allergic rhinitis

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dc.contributor.authorÖZEN, AHMET OĞUZHAN
dc.contributor.authorsOgulur, Ismail; Kiykim, Ayca; Baris, Safa; Ozen, Ahmet; Yuce, Ezgi Gizem; Karakoc-Aydiner, Elif
dc.date.accessioned2022-03-12T20:30:48Z
dc.date.available2022-03-12T20:30:48Z
dc.date.issued2017
dc.description.abstractObjective: Flow cytometric quantification of in vitro basophil activation can be quite performant and reliable tool to measure IgE-dependent allergen-specific responses in allergic patients. Current study aimed to evaluate the clinical relevance of basophil activation test (BAT) for the diagnosis of pediatric grass pollen and house dust mite (HDM) allergies. Methods: Forty-seven patients suffering from allergic rhinitis with HDM and grass pollen co sensitization with clinical history of allergic rhinitis and/or asthma and 15 non-allergic healthy subjects were enrolled. BAT was determined by flow cytometry upon double staining with anti-IgE/anti-CD63 mAb. Results: Regarding HDM with cut-off point greater than 12.5% for CD63(+) basophils sensitivity and specificity of the BAT were 90% and 73%, with positive predictive value (PPV) and negative predictive value (NPV) as 0.70 and 0.91, respectively. The analysis of concordance of being either allergic or healthy in comparison to BAT results for HDM revealed a substantial concordance (K index = 0.61, p < 0.001). Grass pollen with cut-off point greater than 11%, BAT attained a sensitivity, specificity, PPV, and NPV of 96%, 93%, 0.98, and 0.88, respectively. The analysis of concordance of being either allergic or healthy in comparison to BAT results for grass pollen revealed an almost perfect concordance (K index = 0.87, p < 0.001). Conclusion: Our findings concluded that BAT is reliable technique in the diagnosis of sensitization to grass pollen and HDM. The sensitivity of BAT in pollen allergic children was found to be remarkably higher in our cohort compared to other studies. (C) 2017 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.ijporl.2017.04.006
dc.identifier.eissn1872-8464
dc.identifier.issn0165-5876
dc.identifier.pubmed28483235
dc.identifier.urihttps://hdl.handle.net/11424/234212
dc.identifier.wosWOS:000403730300033
dc.language.isoeng
dc.publisherELSEVIER IRELAND LTD
dc.relation.ispartofINTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBasophil activation test
dc.subjectCD63
dc.subjectFlow cytometer
dc.subjectGrass pollen
dc.subjectHouse dust mite
dc.subjectSensitivity
dc.subjectIMMUNOGLOBULIN-E
dc.subjectPOLLEN ALLERGY
dc.subjectFLOW-CYTOMETRY
dc.subjectRELEASE TESTS
dc.subjectGRASS-POLLEN
dc.subjectEXPRESSION
dc.subjectDIAGNOSIS
dc.subjectPERSPECTIVES
dc.subjectSENSITIVITY
dc.subjectASTHMA
dc.titleBasophil activation test for inhalant allergens in pediatric patients with allergic rhinitis
dc.typearticle
dspace.entity.typePublication
local.avesis.idc514e23c-2b4b-4edc-9d86-316c28ceaaff
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages5
local.journal.quartileQ3
oaire.citation.endPage201
oaire.citation.startPage197
oaire.citation.titleINTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY
oaire.citation.volume97
relation.isAuthorOfPublication3e9c297b-e636-4836-8f61-dc9c8b7c29cf
relation.isAuthorOfPublication.latestForDiscovery3e9c297b-e636-4836-8f61-dc9c8b7c29cf

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