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An analytical framework combining online high-performance liquid chromatography methodologies and biological properties of different extracts of Leonurus cardiaca

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dc.contributor.authorŞENKARDEŞ, İSMAİL
dc.contributor.authorsNilofar N., EYUPOĞLU O. E., Nazzaro F., Fratianni F., Ahmed S., Ferrante C., ŞENKARDEŞ İ., Zengin G.
dc.date.accessioned2023-12-11T08:12:16Z
dc.date.available2023-12-11T08:12:16Z
dc.date.issued2023-01-01
dc.description.abstractLittle or no information is available concerning online high-performance liquid chromatography (HPLC) antioxidants and the antibiofilm effect of Leonurus cardiaca. Five distinct extractions of methanolic, ethyl acetate, dichloromethane, hexane, and water were obtained from L. cardiaca. In the online-HPLC-antioxidant analysis of all examined samples, rosmarinic acid emerged as the primary antioxidant, registering concentrations ranging from 6 to 15 ppm at wavelengths of 517 and 734 nm. Notably, the water extract exhibited robust antioxidant activity In vitro. Regarding acetylcholinesterase and butrylcholinesterase inhibition, the n-hexane extract exhibited superior inhibition with values of 3.08 and 5.83 galanthamine equivalent, respectively. Except for the water extract, all tested extracts (at a concentration of 20 μg/mL) exhibited substantial inhibitory activity against biofilm formation, in many cases superior to 80%, and reached even 94.52% against Escherichia coli. Although less vigorous, the extracts also acted against the mature biofilm (inhibition up 76.50% against Staphylococcus aureus). They could work against the metabolism inside an immature and mature biofilm, with inhibition percentages up to 93.18% (vs. Pseudomonas aeruginosa) and 76.50% (vs. Acinetobacter baumannii), respectively. Considering its significant antioxidants, enzyme inhibition, and antimicrobial activity, L. cardiaca emerges as a promising candidate for therapeutic potential.
dc.identifier.citationNilofar N., EYUPOĞLU O. E., Nazzaro F., Fratianni F., Ahmed S., Ferrante C., ŞENKARDEŞ İ., Zengin G., "An analytical framework combining online high-performance liquid chromatography methodologies and biological properties of different extracts of Leonurus cardiaca", Journal of Separation Science, 2023
dc.identifier.doi10.1002/jssc.202300695
dc.identifier.issn1615-9306
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85178446604&origin=inward
dc.identifier.urihttps://hdl.handle.net/11424/295506
dc.language.isoeng
dc.relation.ispartofJournal of Separation Science
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectKimya
dc.subjectAnalitik Kimya
dc.subjectTemel Bilimler
dc.subjectChemistry
dc.subjectAnalytical Chemistry
dc.subjectNatural Sciences
dc.subjectTemel Bilimler (SCI)
dc.subjectKİMYA, ANALİTİK
dc.subjectNatural Sciences (SCI)
dc.subjectCHEMISTRY
dc.subjectCHEMISTRY, ANALYTICAL
dc.subjectFizik Bilimleri
dc.subjectFiltrasyon ve Ayırma
dc.subjectPhysical Sciences
dc.subjectFiltration and Separation
dc.subjectbiofilm
dc.subjectbiological potential
dc.subjectchemical profiles
dc.subjectLeonurus
dc.subjectnatural agents
dc.subjectrosmarinic acid
dc.titleAn analytical framework combining online high-performance liquid chromatography methodologies and biological properties of different extracts of Leonurus cardiaca
dc.typearticle
dspace.entity.typePublication
local.avesis.idd9d22ea2-57a6-42fe-97a7-9f8663dfd369
local.indexed.atPUBMED
local.indexed.atSCOPUS
relation.isAuthorOfPublication3e59035c-d7b1-4887-b78c-2a9bc207de05
relation.isAuthorOfPublication.latestForDiscovery3e59035c-d7b1-4887-b78c-2a9bc207de05

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