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A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection

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dc.contributor.authorŞİŞMAN, ALPER
dc.contributor.authorsOnen, Onursal; Sisman, Alper; Gallant, Nathan D.; Kruk, Patricia; Guldiken, Rasim
dc.date.accessioned2022-03-14T10:17:44Z
dc.date.available2022-03-14T10:17:44Z
dc.date.issued2012-05-31
dc.description.abstractIn this study, the design, fabrication, surface functionalization and experimental characterization of an ultrasonic MEMS biosensor for urinary anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) detection with sub ng/mL sensitivity is presented. It was previously shown that urinary Bcl-2 levels are reliably elevated during early and late stages of ovarian cancer. Our biosensor uses shear horizontal (SH) surface acoustic waves (SAWs) on surface functionalized ST-cut Quartz to quantify the mass loading change by protein adhesion to the delay path. SH-SAWs were generated and received by a pair of micro-fabricated interdigital transducers (IDTs) separated by a judiciously designed delay path. The delay path was surface-functionalized with monoclonal antibodies, ODMS, Protein A/G and Pluronic F127 for optimal Bcl-2 capture with minimal non-specific adsorption. Bcl-2 concentrations were quantified by the resulting resonance frequency shift detected by a custom designed resonator circuit. The target sensitivity for diagnosis and identifying the stage of ovarian cancer was successfully achieved with demonstrated Bcl-2 detection capability of 500 pg/mL. It was also shown that resonance frequency shift increases linearly with increasing Bcl-2 concentration.
dc.identifier.doi10.3390/s120607423
dc.identifier.issn1424-8220
dc.identifier.pubmed22969352
dc.identifier.urihttps://hdl.handle.net/11424/244316
dc.identifier.wosWOS:000305801400032
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofSENSORS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectovarian cancer
dc.subjectMEMS
dc.subjectpiezoelectric
dc.subjectsensor
dc.subjectearly detection
dc.subjectBcl-2
dc.subjectSH
dc.subjectSAW
dc.subjectSAM
dc.subjectultrasound
dc.subjectESCHERICHIA-COLI
dc.subjectPROPAGATION
dc.subjectBIOMARKERS
dc.subjectDISCOVERY
dc.subjectEFFICACY
dc.subjectSENSORS
dc.subjectBIOLOGY
dc.subjectLINBO3
dc.subjectWOMEN
dc.subjectSTAGE
dc.titleA Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection
dc.typearticle
dspace.entity.typePublication
local.avesis.idc7710e9a-979c-4bd7-8655-7c5e9d773340
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages15
oaire.citation.endPage7437
oaire.citation.issue6
oaire.citation.startPage7423
oaire.citation.titleSENSORS
oaire.citation.volume12
relation.isAuthorOfPublication26706459-fa25-48f4-8e21-7b1560c9b9d7
relation.isAuthorOfPublication.latestForDiscovery26706459-fa25-48f4-8e21-7b1560c9b9d7

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