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IFN-gamma stimulation of dental follicle mesenchymal stem cells modulates immune response of CD4(+) T lymphocytes in Der p1(+) asthmatic patients in vitro

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dc.contributor.authorAKKOÇ, TUNÇ
dc.contributor.authorsGenc, D.; Zibandeh, N.; Nain, E.; Arig, U.; Goker, K.; Aydiner, E. K.; Akkoc, T.
dc.date.accessioned2022-03-12T22:29:44Z
dc.date.available2022-03-12T22:29:44Z
dc.date.issued2019
dc.description.abstractBackground: House dust mite (Dermataphagoides pteronyssinus) is a widespread risk factor in the development of asthma. CD4(+) T lymphocytes have an important role in the pathogenesis of allergic asthma by polarizing to Th2 cells. Objective: We aimed to evaluate the immunoregulatory effects of dental follicle mesenchymal stem cells with and without IFN-gamma stimulation on peripheral blood mononuclear cells of house dust mite sensitive asthmatic patients, and compared those with Dexamethasone as a systemic steroid. Material and methods: PBMC of asthmatic patients and healthy individuals separately cultured with or without DF-MSCs in the presence and absence of IFN-gamma or Der p1 or Dexamethasone for 72 h. CD4(+) T proliferation, cell viability, CD4(+)CD25(+)FoxP3(+) Treg cell frequency and cytokine profiles of PBMC were evaluated via flow cytometry. Results: DF-MSCs suppressed proliferation of CD4(+) T lymphocytes (p(CDmix) < 0.01, p(Derp1) < 0.01, p(IFN) < 0.005) by increasing the number of FoxP3 expressing CD4(+)CD25(+) T regulatory cells (p(CDmix) < 0.005, p(Derp1) < 0.01, p(IFN) < 0.001) and suppressed lymphocyte apoptosis (p(CDmix) < 0.05, p(Derp1) < 0.05, p(IFN) < 0.05), while Dexamethasone increased the apoptosis and decreased Treg cell frequency in asthmatic patients. IFN-gamma stimulation increased the suppressive effect of DF-MSCs and also enhanced the frequency of FoxP3 expressing CD4(+)CD25(+) T regulatory cells. The cytokine levels were regulated by DF-MSCs by reducing IL-4 cytokine levels (p(CDmix) < 0.01, p(Derp1) < 0.05, p(IFN) < 0.05) and upregulating IFN-gamma levels (p(CDmix) < 0.01, p(Derp1) < 0.05, p(IFN) < 0.005) in asthmatic patients. Conclusion: IFN-gamma stimulated DF-MSCs were found to have a high modulatory effect on CD4(+) T cell responses, while Dexamethasone had an apoptotic effect on CD4(+) T cells in asthmatic patients. DF-MSCs may be a new cell-based therapy option for allergic diseases including asthma. (C) 2019 SEICAP. Published by Elsevier Espana, S.L.U. All rights reserved.
dc.identifier.doi10.1016/j.aller.2018.12.005
dc.identifier.eissn1578-1267
dc.identifier.issn0301-0546
dc.identifier.pubmed30826066
dc.identifier.urihttps://hdl.handle.net/11424/235405
dc.identifier.wosWOS:000486736100009
dc.language.isoeng
dc.publisherELSEVIER ESPANA SLU
dc.relation.ispartofALLERGOLOGIA ET IMMUNOPATHOLOGIA
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDental follicle mesenchymal stem cells
dc.subjectAsthma
dc.subjectHouse dust mite
dc.subjectDer p1
dc.subjectINTERFERON-GAMMA
dc.subjectCOCKROACH ALLERGEN
dc.subjectINFLAMMATION
dc.subjectEPITHELIUM
dc.subjectPATHOLOGY
dc.subjectEXPOSURE
dc.subjectCHILDREN
dc.titleIFN-gamma stimulation of dental follicle mesenchymal stem cells modulates immune response of CD4(+) T lymphocytes in Der p1(+) asthmatic patients in vitro
dc.typearticle
dspace.entity.typePublication
local.avesis.idf0afb932-dfd3-478c-9855-7b46b0baf060
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages10
local.journal.quartileQ4
oaire.citation.endPage476
oaire.citation.issue5
oaire.citation.startPage467
oaire.citation.titleALLERGOLOGIA ET IMMUNOPATHOLOGIA
oaire.citation.volume47
relation.isAuthorOfPublicationdde3c531-d82d-492a-96eb-d6724483eaa1
relation.isAuthorOfPublication.latestForDiscoverydde3c531-d82d-492a-96eb-d6724483eaa1

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