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Live Cell Imaging of Peptide Uptake Using a Microfluidic Platform

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsYuce, Merve; Ozkirimli, Elif; Sariyar Akbulut, Berna; Ulgen, Kutlu
dc.date.accessioned2022-03-12T22:57:52Z
dc.date.available2022-03-12T22:57:52Z
dc.date.issued2021
dc.description.abstractCell penetrating peptides (CPPs) are unique molecules with the ability to pass through biological membranes as they carry their cargoes into the cells. Endocytosis and direct penetration have been proposed as the two major mechanisms for their internalization. However, the involvement of oligo peptide permease (Opp) in bacterial CPP uptake is an issue that yet remains to be elucidated. Here, a microfluidic platform coupled with live cell imaging was used to monitor CPP uptake in Escherichia coli. The set-up enabled imaging on a single-cell basis, helped to capture cell dynamics at a micro-scale and provided quantitative real-time information. The constructed platform also provided evidence on the contribution of Opp to peptide uptake. Additional high-resolution visualization under confocal microscopy confirmed that in the absence of Opp, the internalized peptides accumulated in the periplasm. Here, we have shown that microfluidic platforms, which have successful applications in different areas, can be used as a highly reliable and versatile tool for studying peptide uptake in bacterial cells.
dc.identifier.doi10.1007/s10989-021-10228-6
dc.identifier.eissn1573-3904
dc.identifier.issn1573-3149
dc.identifier.urihttps://hdl.handle.net/11424/237109
dc.identifier.wosWOS:000652906400001
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofINTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMicrofluidics
dc.subjectCell penetrating peptides
dc.subjectPeptide uptake
dc.subjectLive cell monitoring
dc.subjectE
dc.subjectcoli
dc.subjectPENETRATING PEPTIDES
dc.subjectMECHANISMS
dc.titleLive Cell Imaging of Peptide Uptake Using a Microfluidic Platform
dc.typearticle
dspace.entity.typePublication
local.avesis.id3dbc7958-0575-4c56-968c-5c3f3e0bb9ef
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages11
oaire.citation.endPage2013
oaire.citation.issue3
oaire.citation.startPage2003
oaire.citation.titleINTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
oaire.citation.volume27
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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