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Evaluation of two commercial methods for rapid detection of the carbapenemase-producing Klebsiella pneumoniae

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dc.contributor.authorALTINKANAT GELMEZ, GÜLŞEN
dc.contributor.authorsGelmez, Gulsen Altinkanat; Can, Baris; Hasdemir, Ufuk; Soyletir, Guner
dc.date.accessioned2022-03-12T22:40:36Z
dc.date.available2022-03-12T22:40:36Z
dc.date.issued2020
dc.description.abstractIn this study, we evaluated the performance of the two commercial methods (Rapidec Carba NP and NG-Test Carba-5) for the rapid detection of carbapenemase-producing Klebsiella pneumoniae. A total of 224 Klebsiella pneumoniae strains previously characterized for carbapenemase genes by polymerase chain reaction were included in the study. The strain collection included 30 non-carbapenemase producers, 85 OXA-48-like, 59 NDM, 14 IMP, 7 KPC, 7 VIM, 19 OXA-48-like plus NDM, and 3 KPC plus NDM producers. Rapidec Carba NP and NG-Test Carba 5 was performed according to the manufacturer's instructions. NG-Test Carba 5 correctly detected all carbapenemase-producing K. pneumoniae, however, Rapidec Carba NP failed to detect 41% of OXA-48-like producers even with extended incubation time. The overall sensitivity and specificity were 81,9% and 100% for Rapidec Carba NP, 100% and 100% for NG-Test Carba 5, respectively. Both tests seem to be fast and reliable for the detection of carbapenemase-producing K. pneumoniae especially for microbiology laboratories where molecular tests cannot be performed. However, Rapidec Carba NP with weak hydrolysis activity for OXA-48 like might be used in regions where OXA-48 is not prevalent. The additional advantage of NG-Test Carba 5 is that it specifically detects carbapenemases giving way to threat-related infections with an effective drug such as ceftazidime-avibactam or meropenem- vaborbactam.
dc.identifier.doi10.1016/j.mimet.2020.106084
dc.identifier.eissn1872-8359
dc.identifier.issn0167-7012
dc.identifier.pubmed33049329
dc.identifier.urihttps://hdl.handle.net/11424/235988
dc.identifier.wosWOS:000589897300005
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofJOURNAL OF MICROBIOLOGICAL METHODS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectRapidec Carba NP
dc.subjectNG-test Carba 5
dc.subjectCarbapenemase-producing Klebsiella pneumoniae
dc.subjectCARBA NP TEST
dc.subjectDESORPTION IONIZATION-TIME
dc.subjectIMMUNOCHROMATOGRAPHIC ASSAYS
dc.subjectPSEUDOMONAS-AERUGINOSA
dc.subjectENTEROBACTERIACEAE
dc.subjectOXA-48
dc.subjectTESTS
dc.subjectPREVALENCE
dc.subjectSTRATEGIES
dc.subjectRESISTANCE
dc.titleEvaluation of two commercial methods for rapid detection of the carbapenemase-producing Klebsiella pneumoniae
dc.typearticle
dspace.entity.typePublication
local.avesis.idf349dc7a-1c1c-4d67-a6af-b09bffb8e22a
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumber106084
local.journal.numberofpages6
local.journal.quartileQ3
oaire.citation.titleJOURNAL OF MICROBIOLOGICAL METHODS
oaire.citation.volume178
relation.isAuthorOfPublication49c5539e-4c6e-4588-b52c-dcc5cb2979ab
relation.isAuthorOfPublication.latestForDiscovery49c5539e-4c6e-4588-b52c-dcc5cb2979ab

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