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Nesfatin-1 improves oxidative skin injury in normoglycemic or hyperglycemic rats

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dc.contributor.authorYEGEN, BERRAK
dc.contributor.authorsSolmaz, Ali; Bahadir, Elif; Gulcicek, Osman B.; Yigitbas, Hakan; Celik, Atilla; Karagoz, Ayca; Ozsavci, Derya; Sirvanci, Serap; Yegen, Berrak C.
dc.date.accessioned2022-03-12T20:27:54Z
dc.date.available2022-03-12T20:27:54Z
dc.date.issued2016
dc.description.abstractHyperglycemia is one of the major causes of suppressed angiogenesis and impaired wound healing leading to chronic wounds. Nesfatin-1 a novel peptide was reported to have antioxidant and anti-apoptotic properties. This study is aimed to investigate the potential healing-promoting effects of nesfatin-1 in non diabetic or diabetic rats with surgical wounds. In male Sprague-Dawley rats, hyperglycemia was induced by intraperitoneal (ip) injection of streptozotocin (55 mg/kg). Under anesthesia, dorsum skin tissues of normoglycemic (n = 16) and hyperglycemic rats were excised (2 x 2 cm, full-thickness), while control rats (n = 16) had neither hyperglycemia nor wounds. Half of the rats in each group were treated ip with saline, while the others were treated with nesfatin-1 (2 g/kg/day) for 3 days until they were decapitated. Plasma interleukin-1-beta (IL-1 beta), transforming growth factor-beta (TGF-beta-1), IL-6 levels, and dermal tissue malondialdehyde (MDA), glutathione (GSH) levels, myeloperoxidase (MPO) and caspase-3 activity were measured. For histological examination, paraffin sections were stained with hematoxylin-eosin or Masson's trichrome and immunohistochemistry for vascular endothelial growth factor (VEGF) was applied. ANOVA and Student's t-tests were used for statistical analysis. Compared to control rats, skin MPO activity, MDA and caspase-3 levels were increased similarly in saline-treated normo- and hyperglycemic rats. Nesfatin-1 depressed MDA, caspase-3, MPO activity and IL-1 beta with concomitant elevations in dermal GSH and plasma TGF-beta-1 levels. Histopathological examination revealed regeneration of epidermis, regular arrangement of collagen fibers in the dermis and a decrease in VEGF immunoreactivity in the epidermal keratinocytes of nesfatin-1-treated groups. Nesfatin-1 improved surgical wound healing in both normo- and hyperglycemic rats via the suppression of neutrophil recruitment, apoptosis and VEGF activation. (C) 2016 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.peptides.2015.12.006
dc.identifier.eissn1873-5169
dc.identifier.issn0196-9781
dc.identifier.pubmed26829459
dc.identifier.urihttps://hdl.handle.net/11424/233805
dc.identifier.wosWOS:000372718700001
dc.language.isoeng
dc.publisherELSEVIER SCIENCE INC
dc.relation.ispartofPEPTIDES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectNesfatin-1
dc.subjectVascular endothelial growth factor (VEGF)
dc.subjectHyperglycaemia
dc.subjectSurgical wound
dc.subjectNeutrophil infiltration
dc.subjectOxidative injury
dc.subjectENDOTHELIAL GROWTH-FACTOR
dc.subjectFACTOR EXPRESSION
dc.subjectANTIOXIDANT
dc.subjectINSULIN
dc.subjectBETA
dc.subjectIMMUNOREACTIVITY
dc.subjectGLUTATHIONE
dc.subjectSTRESS
dc.subjectULCERS
dc.subjectDAMAGE
dc.titleNesfatin-1 improves oxidative skin injury in normoglycemic or hyperglycemic rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id29334251-7f67-41ed-930b-b8eb28943cdc
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages10
local.journal.quartileQ2
oaire.citation.endPage10
oaire.citation.startPage1
oaire.citation.titlePEPTIDES
oaire.citation.volume78
relation.isAuthorOfPublicatione4eaf9ac-f8dc-4e2b-b940-895cc906790d
relation.isAuthorOfPublication.latestForDiscoverye4eaf9ac-f8dc-4e2b-b940-895cc906790d

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