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Preparation of poly(3-hydroxybutyrate-co-hydroxyvalerate) films from halophilic archaea and their potential use in drug delivery

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dc.contributor.authorOGAN, AYŞE
dc.contributor.authorsDanis, Ozkan; Ogan, Ayse; Tatlican, Pinar; Attar, Azade; Cakmakci, Emrah; Mertoglu, Bulent; Birbir, Meral
dc.date.accessioned2022-03-13T12:50:44Z
dc.date.available2022-03-13T12:50:44Z
dc.date.issued2015
dc.description.abstractHalophilic archaea offer a potential source for production of polyhydroxyalkanoates (PHAs). Hence, the experiments were carried out with five extremely halophilic archaeal isolates to determine the highest PHA-producing strain. PHA production of each isolates was separately examined in cheap carbon sources such as corn starch, sucrose, whey, apple, melon and tomato wastes. Corn starch was found to be a fairly effective substrate for PHA production. Among the strains studied here, the strain with the highest capability for PHA biosynthesis was found to be 1KYS1. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that 1KYS1 closely related to species of the genus Natrinema. The closest phylogenetic similarity was with the strain of Natrinema pallidum JCM 8980 (99 %). PHA content of 1KYS1 was about 53.14 % of the cell dry weight when starch was used as a carbon source. The formation of large and uniform PHA granules was confirmed by transmission electron microscopy and the biopolymer was identified as poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV). PHBV produced by 1KYS1 was blended with low molar mass polyethylene glycol (PEG 300) to prepare biocompatible films for drug delivery. Rifampicin was used as a model drug and its release from PHBV films was investigated at pH 7.4, 37 A degrees C. It was found that PHBV films obtained from 1KYS1 were very effective for drug delivery. In conclusion, PHBV of 1KYS1 may have a potential usage in drug delivery applications.
dc.identifier.doi10.1007/s00792-015-0735-4
dc.identifier.eissn1433-4909
dc.identifier.issn1431-0651
dc.identifier.pubmed25663452
dc.identifier.urihttps://hdl.handle.net/11424/238404
dc.identifier.wosWOS:000350306200026
dc.language.isoeng
dc.publisherSPRINGER JAPAN KK
dc.relation.ispartofEXTREMOPHILES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHalophilic archaea
dc.subjectpoly(3-hydroxybutyrate-co-hydroxyvalerate)
dc.subjectPHBV
dc.subjectDrug delivery
dc.subjectRifampicin
dc.subjectRECOMBINANT ESCHERICHIA-COLI
dc.subjectHALOMONAS-BOLIVIENSIS LC1
dc.subjectBACTERIAL POLYHYDROXYALKANOATES
dc.subjectHALOFERAX-MEDITERRANEI
dc.subjectPOLY(BETA-HYDROXYBUTYRATE) PRODUCTION
dc.subjectPOLY(3-HYDROXYBUTYRIC ACID)
dc.subjectMODERATE HALOPHILE
dc.subjectBIOSYNTHESIS
dc.subjectSPECTROSCOPY
dc.subjectHALOARCULA
dc.titlePreparation of poly(3-hydroxybutyrate-co-hydroxyvalerate) films from halophilic archaea and their potential use in drug delivery
dc.typearticle
dspace.entity.typePublication
local.avesis.id16e5f1ed-6cbd-45b6-8140-4e8250799dce
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages10
oaire.citation.endPage524
oaire.citation.issue2
oaire.citation.startPage515
oaire.citation.titleEXTREMOPHILES
oaire.citation.volume19
relation.isAuthorOfPublication58db3332-a5f8-4224-a021-aeea795f52fa
relation.isAuthorOfPublication.latestForDiscovery58db3332-a5f8-4224-a021-aeea795f52fa

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