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Propylthiouracil (PTU)-induced hypothyroidism alleviates burn-induced multiple organ injury

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dc.contributor.authorVELİOĞLU ÖĞÜNÇ, AYLİZ
dc.contributor.authorsSener, Goeksel; Sehirli, Oezer; Velioglu-Oeguenc, Ayliz; Ercan, Feriha; Erkanli, Goezde; Gedik, Nursal; Yegen, Berrak C.
dc.date.accessioned2022-03-12T17:19:44Z
dc.date.available2022-03-12T17:19:44Z
dc.date.issued2006
dc.description.abstractOxidative stress has an important role in the development of multiorgan failure after major burn. This study was designed to determine the possible protective effect of experimental hypothyroidism in hepatic and gastrointestinal injury induced by thermal trauma. Sprague Dawley rats were administered saline or PTU (10 mg kg(-1) i.p.) for 15 days, and hypothyroidism was confirmed by depressed serum T-3 and T-4 concentrations. Under brief ether anesthesia, shaved dorsurn of rats was exposed to 90 degrees C (burn group) or 25 degrees C (control group) water bath for 10 s. PTU or saline treatment was repeated at the 12th hour of the burn. Rats were decapitated 24 h after injury and tissue samples from liver, stomach and ileum were taken for the determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and collagen contents. Formation of reactive oxygen species in tissue samples was monitored by using chemiluminescence (CL) technique with luminol and lucigenin probes. Tissues were also examined microscopically. Tumor necrosis factor (TNF)-alpha and lactate dehydrogenase (LDH) were assayed in serum samples. Severe skin scald injury (30% of. total body surface area) caused a significant decrease in GSH level, which was accompanied with significant increases in NIDA level, MPO activity, CL levels and collagen content of the studied tissues (p < 0.05-0.001). Similarly, serum TNF-alpha and LDH were elevated in the burn group as compared to control group. On the other hand, PTU treatment reversed all these biochemical indices, as well as histopathological alterations induced by thermal trauma. Our results suggest that PTU-induced hypothyroidism reduces oxidative damage in the hepatic, gastric and ileal tissues probably due to hypometabolism, which is associated with decreased production of reactive oxygen metabolites and enhancement of antioxidant mechanisms. (C) 2006 Elsevier Ltd and ISBI. All rights reserved.
dc.identifier.doi10.1016/j.burns.2006.01.002
dc.identifier.issn0305-4179
dc.identifier.pubmed16926069
dc.identifier.urihttps://hdl.handle.net/11424/228148
dc.identifier.wosWOS:000241271200011
dc.language.isoeng
dc.publisherELSEVIER SCI LTD
dc.relation.ispartofBURNS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPTU
dc.subjectexperimental hypothyroidism
dc.subjectGSH
dc.subjectMPO
dc.subjectlipid peroxidation
dc.subjectLIPID-PEROXIDATION
dc.subjectOXIDATIVE STRESS
dc.subjectTHERMAL-INJURY
dc.subjectFREE-RADICALS
dc.subjectLIVER-MITOCHONDRIA
dc.subjectANTITHYROID DRUGS
dc.subjectREACTIVE OXYGEN
dc.subjectTHYROID STATUS
dc.subjectRAT
dc.subjectMECHANISMS
dc.titlePropylthiouracil (PTU)-induced hypothyroidism alleviates burn-induced multiple organ injury
dc.typearticle
dspace.entity.typePublication
local.avesis.id07b22a09-e053-41e9-ba34-bdd3370156d5
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages9
oaire.citation.endPage736
oaire.citation.issue6
oaire.citation.startPage728
oaire.citation.titleBURNS
oaire.citation.volume32
relation.isAuthorOfPublication13300bf6-ba96-4f87-9868-b0d2c86f572a
relation.isAuthorOfPublication.latestForDiscovery13300bf6-ba96-4f87-9868-b0d2c86f572a

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