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Evaluation of phenotypic tests for detection of carbapenemases: New modifications with new interpretation

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dc.contributor.authorALTINKANAT GELMEZ, GÜLŞEN
dc.contributor.authorsGelmez, Gulsen Altinkanat; Can, Baris; Hasdemir, Ufuk; Soyletir, Guner
dc.date.accessioned2022-03-12T22:55:19Z
dc.date.available2022-03-12T22:55:19Z
dc.date.issued2021
dc.description.abstractIntroduction: The emergence and spread of carbapenemase-producing Enterobacterales (CPE) is a worldwide public health threat. Rapid and accurate detection of CPE is essential to prevent their dissemination within health care settings. The aim of this study was to evaluate the performance of CIM, mCIM and mCIM with ammonium bicarbonate (mCIM-A) methods by using different interpretation criteria for detection of carbapenemases. Methods: One hundred and fifty-three Klebsiella pneumoniae isolates previously characterized by molecular tests, including 133 carbapenemase producers and 20 non-carbapenemase producers, were collected in this study. CIM and mCIM tests were performed as described previously. mCIM-A by adding 50 mM ammonium bicarbonate to the bacterial suspension prepared in tryptic soy broth. The inhibition zone diameter of around meropenem disc was measured and interpreted as positive according to i) Pierce and colleagues (<19 mm), ii) EUCAST meropenem susceptibility breakpoint (<22). Results: CIM, although seems to be good for carbapenemases other than OXA-48-like and NDM, is not satisfactory (42.3% and 83.4%, respectively) for those enzymes with any of the interpretation criteria. OXA-48-like and NDM were detected with a better performance (88.7% and 92.8, respectively) with mCIM when results were interpreted according to <22 mm zone diameter for OXA-48-like and NDM. The best results were obtained with mCIM-A using <22 mm criteria without any difference in the results of other enzymes and negative strains. Conclusions: mCIM- A method interpreted with <22 mm meropenem zone diameter seems to be preferable compared to CIM and mCIM. mCIM-A is simple and useful tool for identification of CPEs in clinical microbiology laboratories. (C) 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.jiac.2020.09.021
dc.identifier.eissn1437-7780
dc.identifier.issn1341-321X
dc.identifier.pubmed33008736
dc.identifier.urihttps://hdl.handle.net/11424/236713
dc.identifier.wosWOS:000614051100014
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofJOURNAL OF INFECTION AND CHEMOTHERAPY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCarbapenem inactivation method
dc.subjectModified carbapenem inactivation method
dc.subjectAmmonium bicarbonate
dc.subjectCarbapenemase-producing klebsiella pneumoniae
dc.titleEvaluation of phenotypic tests for detection of carbapenemases: New modifications with new interpretation
dc.typearticle
dspace.entity.typePublication
local.avesis.idc91c6d12-a7cc-43be-94e6-197ea5ca1eb2
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage231
oaire.citation.issue2
oaire.citation.startPage226
oaire.citation.titleJOURNAL OF INFECTION AND CHEMOTHERAPY
oaire.citation.volume27
relation.isAuthorOfPublication49c5539e-4c6e-4588-b52c-dcc5cb2979ab
relation.isAuthorOfPublication.latestForDiscovery49c5539e-4c6e-4588-b52c-dcc5cb2979ab

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