Publication:
Thermostable alpha-amylase from moderately halophilic Halomonas sp AAD21

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsUzyol, Kamil Serkan; Akbulut, Berna Sariyar; Denizci, Aziz Akin; Kazan, Dilek
dc.date.accessioned2022-03-12T18:06:07Z
dc.date.available2022-03-12T18:06:07Z
dc.date.issued2012
dc.description.abstractThe moderately halophilic Halomonas sp. strain AAD21, which produces extracellular thermostable alpha-amylase, was isolated from the Camalti Saltern area located in Izmir, Turkey. NaCl, carbon, and nitrogen sources in the growth medium were optimized to enhance alpha-amylase yield. The highest enzyme yield was measured in the presence of 20% NaCl with peptone as the nitrogen and starch as the carbon sources in the fermentation broth. This microorganism was also found to utilize waste potato peel as a carbon source for alpha-amylase production. Concentrations of carbon and nitrogen sources were optimized using a statistical approach, and alpha-amylase activity increased from 4.07 U mL(-1) min(-1) to 26.25 U mL(-1) min(-1). Maximum alpha-amylase production was achieved at the end of 48 h of growth in the presence of 20% NaCl, 4.12% starch, 1.0% peptone, 0.2% KCl, 2% MgSO4 center dot 7H(2)O, and 0.03% trisodium citrate pentahydrate. The optimum pH and temperature of the alpha-amylase were found to be 7.0 and 50 degrees C, respectively. The alpha-amylase synthesized by Halomonas sp. AAD21 was also thermostable. Crude alpha-amylase did not lose its original activity after 2 h of incubation at 50 degrees C and 60 degrees C, and it retained 70% of its original activity after 120 min of incubation at 90 degrees C.
dc.identifier.doi10.3906/biy-1106-7
dc.identifier.eissn1303-6092
dc.identifier.issn1300-0152
dc.identifier.urihttps://hdl.handle.net/11424/230840
dc.identifier.wosWOS:000304071800011
dc.language.isoeng
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
dc.relation.ispartofTURKISH JOURNAL OF BIOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectModerate halophile
dc.subjectHalomonas sp AAD21
dc.subjectalpha-amylase
dc.subjectthermostable
dc.subjectoptimization
dc.subjectXYLANASE PRODUCTION
dc.subjectPURIFICATION
dc.titleThermostable alpha-amylase from moderately halophilic Halomonas sp AAD21
dc.typearticle
dspace.entity.typePublication
local.avesis.id654c3e98-2385-471c-a0d2-1b3d3457ec7a
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages12
oaire.citation.endPage338
oaire.citation.issue3
oaire.citation.startPage327
oaire.citation.titleTURKISH JOURNAL OF BIOLOGY
oaire.citation.volume36
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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