Publication:
Potential Effect of 1,25 Dihydroxyvitamin D-3 on Thioacetamide-Induced Hepatotoxicity in Rats

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dc.contributor.authorYÜKSEL, MERAL
dc.contributor.authorsOzdemir-Kumral, Zarife N.; Erkek, Burak E.; Karakus, Buse; Almaci, Meslina; Fathi, Reza; Yuksel, Meral; Cumbul, Alev; Alican, Inci
dc.date.accessioned2022-03-12T22:38:15Z
dc.date.available2022-03-12T22:38:15Z
dc.date.issued2019
dc.description.abstractBackground: 1,25 Dihydroxyvitamin D-3 (1,25(OH)(2)D-3) modulates inflammation and immune responses. Deficiency of 1,25(OH)(2)D-3 was found to be associated with the risk of cancer, cardiovascular disease, osteoarthritis, infections, and autoimmune diseases. This study evaluated the effect of 1,25 dihydroxyvitamin D-3 1,25(OH)(2)D-3 on thioacetamide (TAA)-induced acute liver injury in rats. Materials and methods: Rats were treated with either saline or 1,25(OH)(2)D-3 (0.30 mg/kg; orogastrically) for 15 d. Starting from day 13, TAA (200 mg/kg; intraperitoneally) was given for 3 d. On day 15, all rats were euthanized. Liver and blood samples were collected. Results: TAA caused severe damage, increased lipid peroxidation with reductions in endogenous antioxidants, increased apoptosis, increased production of reactive oxygen species, and elevated inducible nitric oxide synthase (iNOS), and nuclear factor kappa B (NF-kappa B) expression in liver. Extent of damage was decreased by 1,25(OH)(2)D-3 (P < 0.01). 1,25(OH)(2)D-3 attenuated the increase in malondialdehyde (P < 0.01), increase in myeloper-oxidase (P < 0.01), increase in chemiluminescence levels (P < 0.05) and apoptotic activity (P < 0.001). Elevated liver iNOS and NF-kappa B expression in TAA group was also reduced by 1,25(OH)(2)D-3 (P < 0.001, for iNOS; P < 0.001, for NF-kappa B). TAA group revealed high serum aspartate transaminase and alanine transaminase (ALT) activities (P < 0.01, for aspartate transaminase; P = 0.08, for ALT) and reduced albumin levels (P < 0.01) compared with control. 1,25(OH)(2)D-3 had no statistically significant effect on these parameters. Conclusions: 1,25(OH)(2)D-3 provides protection against hepatic injury in a rat model of TAA-induced hepatotoxicity via suppression of inflammatory reaction, oxidative stress, and apoptosis. (C) 2019 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.jss.2019.05.020
dc.identifier.eissn1095-8673
dc.identifier.issn0022-4804
dc.identifier.pubmed31177036
dc.identifier.urihttps://hdl.handle.net/11424/235564
dc.identifier.wosWOS:000488045900025
dc.language.isoeng
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.ispartofJOURNAL OF SURGICAL RESEARCH
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosis
dc.subject1,25(OH)(2)D-3
dc.subjectHepatotoxicity
dc.subjectOxidative stress
dc.subjectRat
dc.subjectThioacetamide
dc.subjectINDUCED LIVER-INJURY
dc.subjectVITAMIN-D
dc.subjectHEPATIC-ENCEPHALOPATHY
dc.subjectNITRIC-OXIDE
dc.subjectOXIDATIVE STRESS
dc.subjectAPOPTOSIS
dc.subjectPROTECTS
dc.subjectINFLAMMATION
dc.subjectINVOLVEMENT
dc.subjectMODELS
dc.titlePotential Effect of 1,25 Dihydroxyvitamin D-3 on Thioacetamide-Induced Hepatotoxicity in Rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id399c9787-f061-4016-a032-4b68e4931048
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages8
local.journal.quartileQ3
oaire.citation.endPage172
oaire.citation.startPage165
oaire.citation.titleJOURNAL OF SURGICAL RESEARCH
oaire.citation.volume243
relation.isAuthorOfPublication8b13d479-2f3b-4180-bc71-7ad5a5625f1b
relation.isAuthorOfPublication.latestForDiscovery8b13d479-2f3b-4180-bc71-7ad5a5625f1b

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