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Flow cytometry analysis of guided tissue regeneration-associated human periodontal cells

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dc.contributor.authorKURU, LEYLA
dc.contributor.authorsKuru, L; Parkar, MH; Griffiths, GS; Olsen, I
dc.date.accessioned2022-03-12T17:01:16Z
dc.date.available2022-03-12T17:01:16Z
dc.date.issued2001
dc.description.abstractBackground: Expanded polytetrafluoroethylene (ePTFE) barrier membranes have been widely used for guided tissue regeneration (GTR) of the human periodontal ligament (PL). However, the precise cellular and molecular events involved in the re-growth of the new tissue are still unclear. Methods: Retrieved membranes and the newly -regenerated soft tissue (RT) underlying the membranes were used to examine the cells associated with GTR compared with normal human PL and gingival cells. Flow cytometry (FCM) was used, for the first time, to analyze the spindle-shaped fibroblast-like cells which were adherent to these membranes and the cells which grew out of the RT. Results: The results showed that the membrane-associated (M) cells had the lowest rate of proliferation and appeared to be larger and more granular than the other types of cell. Moreover, both the M- and RT-derived cells were found to express higher levels of the extracellular matrix (ECM) proteins Collagen type I, fibronectin, tenascin, and decorin. In addition, evidence based on FCM profiles identified distinct sub-populations of GTR cells in which fibronectin expression was markedly up-regulated compared with normal PL cells and which also differed in size and granularity. Conclusions: The results of this study show that cells associated with GTR barrier membranes and with the underlying tissue appear to have distinct phenotypic and functional activities consistent with the production of new periodontal connective tissue and periodontal regeneration.
dc.identifier.doi10.1902/jop.2001.72.8.1016
dc.identifier.eissn1943-3670
dc.identifier.issn0022-3492
dc.identifier.pubmed11525432
dc.identifier.urihttps://hdl.handle.net/11424/227364
dc.identifier.wosWOS:000170546900005
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofJOURNAL OF PERIODONTOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectmembranes, artificial
dc.subjectmembranes, barrier
dc.subjectguided tissue regeneration
dc.subjectpolytetrafluoroethylene/therapeutic use
dc.subjectperiodontal ligament
dc.subjectfibroblasts
dc.subjectflow cytometry/utilization
dc.subjectEXPANDED POLYTETRAFLUOROETHYLENE MEMBRANES
dc.subjectCONTROLLED CLINICAL-TRIAL
dc.subjectHUMAN INTRABONY DEFECTS
dc.subjectFIBROBLAST SUBPOPULATIONS
dc.subjectBARRIER MEMBRANES
dc.subjectLIGAMENT CELLS
dc.subjectEXPRESSION
dc.subjectGINGIVAL
dc.subjectBONE
dc.subjectHETEROGENEITY
dc.titleFlow cytometry analysis of guided tissue regeneration-associated human periodontal cells
dc.typearticle
dspace.entity.typePublication
local.avesis.id537d03f2-1dbf-4a04-8d48-fd2a6c98d2fc
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages9
oaire.citation.endPage1024
oaire.citation.issue8
oaire.citation.startPage1016
oaire.citation.titleJOURNAL OF PERIODONTOLOGY
oaire.citation.volume72
relation.isAuthorOfPublicationcc49fe59-fb00-4a6a-a32c-60d68c5f3993
relation.isAuthorOfPublication.latestForDiscoverycc49fe59-fb00-4a6a-a32c-60d68c5f3993

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