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Alpha-Amylase Immobilization on Epoxy Containing Thiol-Ene Photocurable Materials

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dc.contributor.authorÇAKMAKÇI, EMRAH
dc.contributor.authorsCakmakci, Emrah; Danis, Ozkan; Demir, Serap; Mulazim, Yusuf; Kahraman, Memet Vezir
dc.date.accessioned2022-03-12T18:07:52Z
dc.date.available2022-03-12T18:07:52Z
dc.date.issued2013
dc.description.abstractThiol-ene polymerization is a versatile tool for several applications. Here we report the preparation of epoxide groups containing thiol-ene photocurable polymeric support and the covalent immobilization of alpha-amylase onto these polymeric materials. The morphology of the polymeric support was characterized by scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) coupled with SEM was used to explore the chemical composition. The polymeric support and the immobilization of the enzyme were characterized by FTIR analysis. SEM-EDS and FTIR results showed that the enzyme was successfully covalently attached to the polymeric support. The immobilization efficiency and enzyme activity of alpha-amylase were examined at various pH (5.0-8.0) and temperature (30-80 degrees C) values. The storage stability and reusability of immobilized alpha-amylase were investigated. The immobilization yield was 276 +/- 1.6 mg per gram of polymeric support. Enzyme assays demonstrated that the immobilized enzyme exhibited better thermostability than the free one. The storage stability and reusability were improved by the immobilization on this enzyme support. Free enzyme lost its activity completely within 15 days. On the other hand, the immobilized enzyme retained 86.7% of its activity after 30 days. These results confirm that alpha-amylase was successfully immobilized and gained a more stable character compared with the free one.
dc.identifier.doi10.4014/jmb.1209.09017
dc.identifier.eissn1738-8872
dc.identifier.issn1017-7825
dc.identifier.pubmed23412063
dc.identifier.urihttps://hdl.handle.net/11424/231069
dc.identifier.wosWOS:000315547800011
dc.language.isoeng
dc.publisherKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
dc.relation.ispartofJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectThiol-ene polymerization
dc.subjectenzyme immobilization
dc.subjectalpha-amylase
dc.subjectUV curing
dc.subjectepoxide functionality
dc.subjectSTARCH-CONVERTING ENZYMES
dc.subjectCOVALENT IMMOBILIZATION
dc.subjectMETHACRYLATE) MICROSPHERES
dc.subjectCA2+ IONS
dc.subjectSURFACE
dc.subjectPHOTOPOLYMERIZATION
dc.subjectPERFORMANCE
dc.subjectSTABILITY
dc.subjectMEMBRANE
dc.subjectKINETICS
dc.titleAlpha-Amylase Immobilization on Epoxy Containing Thiol-Ene Photocurable Materials
dc.typearticle
dspace.entity.typePublication
local.avesis.id4e596311-c12b-4db6-9dcc-8e2d9877a85a
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage210
oaire.citation.issue2
oaire.citation.startPage205
oaire.citation.titleJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
oaire.citation.volume23
relation.isAuthorOfPublication332869cd-e8a9-40ac-b678-cf5b01c6a6dc
relation.isAuthorOfPublication.latestForDiscovery332869cd-e8a9-40ac-b678-cf5b01c6a6dc

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