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Relationship of the cycle threshold values of SARS-CoV-2 polymerase chain reaction and total severity score of computerized tomography in patients with COVID 19

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dc.contributor.authorKARAHASAN, AYŞEGÜL
dc.contributor.authorsYagci, Aysegul Karahasan; Sarinoglu, Rabia Can; Bilgin, Huseyin; Yanilmaz, Ozgur; Sayin, Elvan; Guneser, Deniz; Guncu, Mehmet Mucahit; Doyuk, Zahide; Can, Baris; Kuzan, Beyza Nur; Aslan, Bulent; Korten, Volkan; Cimsit, Cagatay
dc.date.accessioned2022-03-14T10:53:01Z
dc.date.available2022-03-14T10:53:01Z
dc.date.issued2020-12
dc.description.abstractAim: Studies analyzing viral load in COVID-19 patients and any data that compare viral load with chest computerized tomography (CT) severity are limited. This study aimed to evaluate the severity of chest CT in reverse transcriptase polymerase chain reaction (RT-PCR)-positive patients and factors associated with it. Methodology: SARS-CoV-2 RNA was extracted from nasopharyngeal swab samples by using Bio-speedy viral nucleic acid buffer. The RT-PCR tests were performed with primers and probes targeting the RdRp gene (Bioexen LTD, Turkey) and results were quantified as cycle threshold (Ct) values. Chest CT of SARS-CoV-2 RNA-positive patients (n = 730) in a period from 22 March to 20 May 2020 were evaluated. The total severity score (TSS) of chest CT ranged 0-20 and was calculated by summing up the degree of acute lung inflammation lesion involvement of each of the five lung lobes. Results: Of the 284 patients who were hospitalized, 27 (9.5%) of them died. Of 236 (32.3%) patients, there were no findings on CT and 216 (91.5%) of them were outpatients (median age 35 years). TSS was significantly higher in hospitalized patients; 5.3% had severe changes. Ct values were lower among outpatients, indicating higher viral load. An inverse relation between viral load and TSS was detected in both groups. CT severity was related to age, and older patients had higher TSS (p < 0.01). Conclusion: Viral load was not a critical factor for hospitalization and mortality. Outpatients had considerable amounts of virus in their nasopharynx, which made them contagious to their contacts. Viral load is important in detecting early stages of COVID-19, to minimize potential spread, whereas chest CT can help identify cases requiring extensive medical care. (C) 2020 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
dc.identifier.doi10.1016/j.ijid.2020.09.1449
dc.identifier.eissn1878-3511
dc.identifier.issn1201-9712
dc.identifier.pubmed32992013
dc.identifier.urihttps://hdl.handle.net/11424/245270
dc.identifier.wosWOS:000596075200031
dc.language.isoeng
dc.publisherELSEVIER SCI LTD
dc.relation.ispartofINTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSARS-CoV-2
dc.subjectViral load
dc.subjectCT value
dc.subjectChest tomography
dc.subjectTotal severity score
dc.subjectMortality
dc.titleRelationship of the cycle threshold values of SARS-CoV-2 polymerase chain reaction and total severity score of computerized tomography in patients with COVID 19
dc.typearticle
dspace.entity.typePublication
local.avesis.id550e11a0-65b2-45cd-b6d3-4ed6486ca5fd
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
local.journal.quartileQ2
oaire.citation.endPage166
oaire.citation.startPage160
oaire.citation.titleINTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
oaire.citation.volume101
relation.isAuthorOfPublicationd8b6c313-4dc1-4253-adf4-6e2e6d68af90
relation.isAuthorOfPublication.latestForDiscoveryd8b6c313-4dc1-4253-adf4-6e2e6d68af90

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