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Resveratrol ameliorates oxidative DNA damage and protects against acrylamide-induced oxidative stress in rats

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dc.contributor.authorBECEREN, AYFER
dc.contributor.authorsAlturfan, A. Ata; Tozan-Beceren, Ayfer; Sehrili, Ahmet Ozer; Demiralp, Emel; Sener, Goksel; Omurtag, Gulden Zehra
dc.date.accessioned2022-03-12T18:06:06Z
dc.date.available2022-03-12T18:06:06Z
dc.date.issued2012
dc.description.abstractAcrylamide (ACR), used in many fields from industrial manufacturing to laboratory personnel work is also formed during the heating process through interactions of amino acids. Therefore ACR poses a significant risk to human health. This study aimed to elucidate whether resveratrol (RVT) treatment could modulate ACR-induced oxidative DNA damage and oxidative changes in rat brain, lung, liver, kidney and testes tissues. Rats were divided into four groups as control (C); RVT (30 mg/kg i.p. dissolved in 0.9% NaCl), ACR (40 mg/kg i.p.) and RVT + ACR groups. After 10 days rats were decapitated and tissues were excised. 8-hydroxydeoxyguanosine (8-OHdG) is a biomarker of oxidative DNA damage. 8-OHdG content in the extracted DNA solution was determined by enzyme-linked immunosorbent assay method. Malondialdehyde (MDA), glutathione (GSH) levels and myeloperoxidase activity (MPO) were determined in tissues, while oxidant-induced tissue fibrosis was determined by collagen contents. Serum enzyme activities, cytokine levels, leukocyte apoptosis were assayed in plasma. As an indicator of oxidative DNA damage, 8-OHdG levels significantly increased in ACR group and this was reversed significantly by RVT treatment. In ACR group, GSH levels decreased significantly while the MDA levels, MPO activity and collagen content increased in the tissues suggesting oxidative organ damage. In RVT-treated ACR group, oxidant responses reversed significantly. Serum enzyme activities, cytokine levels and leukocyte late apoptosis which increased following ACR administration, decreased with RVT treatment. Therefore supplementing with RVT can be useful in individuals at risk of ACR toxicity.
dc.identifier.doi10.1007/s11033-011-1249-5
dc.identifier.eissn1573-4978
dc.identifier.issn0301-4851
dc.identifier.pubmed21947844
dc.identifier.urihttps://hdl.handle.net/11424/230836
dc.identifier.wosWOS:000301108500140
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofMOLECULAR BIOLOGY REPORTS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcrylamide
dc.subjectResveratrol
dc.subjectOxidative DNA damage
dc.subject8-Hydroxydeoxyguanosine
dc.subjectMyeloperoxidase
dc.subjectLeukocyte apoptosis
dc.subjectNF-KAPPA-B
dc.subjectAPOPTOSIS
dc.subjectCELLS
dc.subjectMYELOPEROXIDASE
dc.subjectEXPRESSION
dc.subjectEXPOSURE
dc.titleResveratrol ameliorates oxidative DNA damage and protects against acrylamide-induced oxidative stress in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id39b43a21-c705-4dc5-aa0f-530d0be0baf7
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages8
oaire.citation.endPage4596
oaire.citation.issue4
oaire.citation.startPage4589
oaire.citation.titleMOLECULAR BIOLOGY REPORTS
oaire.citation.volume39
relation.isAuthorOfPublication759fae37-2af1-47e6-92f8-545c02df272b
relation.isAuthorOfPublication.latestForDiscovery759fae37-2af1-47e6-92f8-545c02df272b

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