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Preparation and antimicrobial properties of LL-37 peptide immobilized lignin/caprolactone polymer film

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dc.contributor.authorOGAN, AYŞE
dc.contributor.authorsOgan, Ayse; Yuce-Dursun, Basak; Abdullah, Deka; Beyler-Cigil, Asli; Kahraman, Memet Vezir; Caglayan, Pinar; Birbir, Meral; Mutlu, Ozal; Gulsoy, Nagihan
dc.date.accessioned2022-03-12T22:41:08Z
dc.date.available2022-03-12T22:41:08Z
dc.date.issued2020
dc.description.abstractThe use of biopolymers has gained priority in tissue engineering and biotechnology, both as dressing material and for enhancing treatment efficiency. There is a demand for new biopolymers designed with protease inhibitors and antimicrobials. LL-37 is an important antimicrobial peptide in human skin and exhibits a broad spectrum of antimicrobial activity against bacteria, fungi, and viral pathogens. Using lignin which is an abundant carbohydrate polymer in nature and a polyacrylic acid, we prepared a lignin/caprolactone biodegradable film by plastifying caprolactone and polyacyrlic acid. Lignin/caprolactone biodegradable film was activated with CDI and then immobilized LL-37 peptide. The structure was elucidated in terms of its functional groups by attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), and the morphology of the lignin/caprolactone biodegradable film was characterized by scanning electron microscopy (SEM) before and after the immobilization process. The amount of LL-37 immobilized was determined by ELISA method. It was found that 97% of LL-37 peptide was successfully immobilized onto the lignin/caprolactone biodegradable film. Antimicrobial activity was determined in the lignin/caprolactone biodegradable film samples by quantitative antimicrobial activity method. According to the results, LL-37 immobilized lignin/caprolactone biodegradable film samples were effective on test organisms; Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. In bio-compatibility assays, the ability to support tissue cell integration was detected by using 3 T3 mouse fibroblasts. Samples were examined under transverse microscope, non-immobilized sample showed a huge cellular death, whereas LL-37 immobilized lignin/caprolactone biodegradable film had identical cellular growth with the control group. This dual functional lignin/caprolactone biodegradable film with enhanced antibacterial properties and increased tissue cell compatibility may be used to design new materials for various types of biological applications.
dc.identifier.doi10.1002/pat.4942
dc.identifier.eissn1099-1581
dc.identifier.issn1042-7147
dc.identifier.urihttps://hdl.handle.net/11424/236072
dc.identifier.wosWOS:000533429200001
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofPOLYMERS FOR ADVANCED TECHNOLOGIES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectantimicrobial activities
dc.subjectbiodegradable film
dc.subjectbiopolymers
dc.subjectlignin
dc.subjectcaprolactone
dc.subjectLL-37 peptide
dc.subjectLIGNIN
dc.subjectMEMBRANE
dc.subjectMECHANISM
dc.subjectCOMPLEX
dc.titlePreparation and antimicrobial properties of LL-37 peptide immobilized lignin/caprolactone polymer film
dc.typearticle
dspace.entity.typePublication
local.avesis.ide96ab01e-c81e-46eb-a3a3-4d3433c08cd0
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages7
local.journal.quartileQ2
oaire.citation.endPage2228
oaire.citation.issue10
oaire.citation.startPage2222
oaire.citation.titlePOLYMERS FOR ADVANCED TECHNOLOGIES
oaire.citation.volume31
relation.isAuthorOfPublication58db3332-a5f8-4224-a021-aeea795f52fa
relation.isAuthorOfPublication.latestForDiscovery58db3332-a5f8-4224-a021-aeea795f52fa

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