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Amine functional magnetic nanoparticles via waterborne thiol-ene suspension photopolymerization for antibody immobilization

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dc.contributor.authorOGAN, AYŞE
dc.contributor.authorsMuhsir, Pelin; Cakmakci, Emrah; Demir, Serap; Ogan, Ayse
dc.date.accessioned2022-03-12T22:24:31Z
dc.date.available2022-03-12T22:24:31Z
dc.date.issued2018
dc.description.abstractThe modification of magnetic nanoparticles (MNPs) via different routes for biomolecule binding is an attractive area of research. Waterborne thiol-ene suspension photopolymerization (TESP) can be a useful method for preparing functional MNPs. In this study, for the very first time waterborne TESP was performed in the presence of MNPs. Neat MNPs were coated and in situ functionalized with amine groups by using thiol-ene chemistry. Engrailed-2 (EN2) protein, a potential biomarker for various cancers such as prostate cancer, bladder cancer, breast cancer and ovarian cancer, is known to be a strong binder to a specific DNA sequence (50-TAATTA-30) to regulate transcription. Anti-EN2 antibodies were immobilized onto these MNPs by physical adsorption and covalent bonding methods, respectively. The amount of the physically immobilized antibodies (0.54 mg/g) were found to be lower than the loading of the covalently bonded antibodies (1.775 mg/g). The biomarker level in the artificial solutions prepared was determined by enzyme-linked immunosorbent assay. Coated MNPs were characterized by FTIR, TGA, SEM and STEM. After TESP, the average diameter of the neat magnetite nanoparticles increased from similar to 15 nm to similar to 32 nm.
dc.identifier.doi10.1016/j.colsurfb.2018.05.062
dc.identifier.eissn1873-4367
dc.identifier.issn0927-7765
dc.identifier.pubmed29894832
dc.identifier.urihttps://hdl.handle.net/11424/234778
dc.identifier.wosWOS:000445989400014
dc.language.isoeng
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofCOLLOIDS AND SURFACES B-BIOINTERFACES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMagnetic nanoparticles
dc.subjectThiol-ene suspension polymerization
dc.subjectAntibody
dc.subjectIRON-OXIDE NANOPARTICLES
dc.subjectPROSTATE-CANCER
dc.subjectCOVALENT IMMOBILIZATION
dc.subjectENGRAILED-2 EN2
dc.subjectSOLID SUPPORTS
dc.subjectCROSS-LINKING
dc.subjectPARTICLE-SIZE
dc.subjectPROTEIN
dc.subjectSURFACE
dc.subjectSTRATEGIES
dc.titleAmine functional magnetic nanoparticles via waterborne thiol-ene suspension photopolymerization for antibody immobilization
dc.typearticle
dspace.entity.typePublication
local.avesis.id92e53094-212f-4cd5-9640-78acfb8b09d5
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
local.journal.quartileQ1
oaire.citation.endPage128
oaire.citation.startPage122
oaire.citation.titleCOLLOIDS AND SURFACES B-BIOINTERFACES
oaire.citation.volume170
relation.isAuthorOfPublication58db3332-a5f8-4224-a021-aeea795f52fa
relation.isAuthorOfPublication.latestForDiscovery58db3332-a5f8-4224-a021-aeea795f52fa

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