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Protective effects of Rubus tereticaulis leaves ethanol extract on rats with ulcerative colitis and bio-guided isolation of its active compounds: A combined in silico, in vitro and in vivo study

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dc.contributor.authorŞEN, ALİ
dc.contributor.authorÖZBEYLİ, DİLEK
dc.contributor.authorERTAŞ, BÜŞRA
dc.contributor.authorDOĞAN, AHMET
dc.contributor.authorBİTİŞ, LEYLA
dc.contributor.authorsŞen A., Özbeyli D., Teralı K., Göger F., Yıldırım A., Ertaş B., Doğan A., Bitiş L., Şener G.
dc.date.accessioned2022-11-18T17:57:44Z
dc.date.available2022-11-18T17:57:44Z
dc.date.issued2022-11-01
dc.description.abstractThe aim of this study was to evaluate the therapeutic effect of active ethanol extract obtained from the leaves of Rubus tereticaulis (RTME) against colitis, and to purify major compounds from this extract by bioassay-directed isolation. Rats with colitis induced via intra-rectal acetic acid administration (5%, v/v) received RTME or sulfasalazine for three consecutive days. On day four, all rats were decapitated, and the colonic tissue samples were collected for macroscopic score, colon weight, reduced glutathione (GSH), myeloperoxidase (MPO), and malondialdehyde (MDA) analyses. The active compounds and chemical composition of RTME were determined by bio-guided isolation and LC-MS/MS, respectively. Compared to the colitis group, the rats treated with RTME displayed significantly lowered macroscopic scores and colon wet weights (p < 0.001). These effects were confirmed biochemically by a decrease in colonic MPO activity (p < 0.001), MDA levels (p < 0.001), and an increase in GSH levels (p < 0.001). Kaempferol-3-O-β-d-glucuronide (RT1) and quercetin-3-O-β-d-glucuronide (RT2) were found to be the major compounds of RTME, as evidenced by in vitro anti-inflammatory and antioxidant activity-guided isolation. Their anti-inflammatory/antioxidant activities were also predicted by docking simulations. Additionally, quinic acid, 5-caffeoylquinic acid, quercetin pentoside, quercetin glucoside, quercetin-3-O-β-d-glucuronide, kaempferol-3-O-β-d-glucuronide, and kaempferol rutinoside were identified in RTME via using LC-MS/MS. RT2, along with other compounds, may be responsible for the observed protective action of RTME against colitis. This study represents the first report on the beneficial effects of RTME in an experimental model of colitis and highlights the potential future use of RTME as a natural alternative to alleviate colitis.
dc.identifier.citationŞen A., Özbeyli D., Teralı K., Göger F., Yıldırım A., Ertaş B., Doğan A., Bitiş L., Şener G., "Protective effects of Rubus tereticaulis leaves ethanol extract on rats with ulcerative colitis and bio-guided isolation of its active compounds: A combined in silico, in vitro and in vivo study", CHEMICO-BIOLOGICAL INTERACTIONS, cilt.1, sa.1, ss.1, 2022
dc.identifier.doi10.1016/j.cbi.2022.110263
dc.identifier.endpage1
dc.identifier.issn0009-2797
dc.identifier.issue1
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1016/j.cbi.2022.110263
dc.identifier.urihttps://hdl.handle.net/11424/283226
dc.identifier.volume1
dc.language.isoeng
dc.relation.ispartofCHEMICO-BIOLOGICAL INTERACTIONS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSağlık Bilimleri
dc.subjectHealth Sciences
dc.subjectKlinik Tıp (MED)
dc.subjectClinical Medicine (MED)
dc.subjectAntioxidant activity
dc.subjectanti-inflammatory activity
dc.subjectcolitis
dc.subjectflavonoids
dc.subjectRubus tereticaulis
dc.titleProtective effects of Rubus tereticaulis leaves ethanol extract on rats with ulcerative colitis and bio-guided isolation of its active compounds: A combined in silico, in vitro and in vivo study
dc.typearticle
dspace.entity.typePublication
local.avesis.id36055c84-447c-4610-afd3-e8942be14ebd
local.indexed.atPUBMED
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relation.isAuthorOfPublication.latestForDiscovery9e088078-d757-41a4-afc6-909f32031cb1

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