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GABA and L-glutamic acid release in en bloc resection slices of human hippocampus: an in vitro microdialysis study

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dc.contributor.authorONAT, FİLİZ
dc.contributor.authorsGoren, MZ; Onat, F; Ozkara, C; Ozyurt, E; Eskazan, E; Aker, R
dc.date.accessioned2022-03-12T15:58:27Z
dc.date.available2022-03-12T15:58:27Z
dc.date.issued2001
dc.description.abstractThe interaction of neurotransmitters has been a major interest in pathophysiological conditions like epilepsy. In vivo microdialysis has recently gained much validity in measuring neurotransmitter release in experimental animals. However, there is a paucity of data concerning its use in humans on the grounds of safety considerations. Microdialysis experiments were performed using die hippocampal head region removed from patients with medically intractable seizures, who underwent surgery for mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS). Following en bloc resection, the tissues were immediately transferred to the essential in vitro milieu. Slices were incubated in lactated Ringer's solution and microdialysis probes inserted into the slices were perfused with artificial cerebrospinal fluid (aCSF). When the K+ concentration of aCSF was elevated to 100 mM, GABA and L-glutamic acid levels increased by 293% and 177%, respectively. This method may serve as an experimental model for human brain, to throw more light on the interactions between GABA and L-glutamic acid in hippocampal tissues obtained from patients with MTLE-HS.
dc.identifier.doi10.1007/s10072-001-8171-x
dc.identifier.eissn1590-3478
dc.identifier.issn1590-1874
dc.identifier.pubmed11808852
dc.identifier.urihttps://hdl.handle.net/11424/224068
dc.identifier.wosWOS:000173311000003
dc.language.isoeng
dc.publisherSPRINGER-VERLAG ITALIA SRL
dc.relation.ispartofNEUROLOGICAL SCIENCES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectin vitro microdialysis
dc.subjectmesial temporal lobe epilepsy
dc.subjectGABA (gamma-aminobutyric acid)
dc.subjectL-glutamic acid
dc.subjecthuman hippocampus
dc.subjectEXTRACELLULAR GABA
dc.subjectAMINO-ACIDS
dc.subjectEPILEPSY
dc.subjectMODEL
dc.subjectRECEPTORS
dc.subjectTHALAMUS
dc.subjectSEIZURE
dc.subjectNEURONS
dc.titleGABA and L-glutamic acid release in en bloc resection slices of human hippocampus: an in vitro microdialysis study
dc.typeconferenceObject
dspace.entity.typePublication
local.avesis.id9d8de85e-b3e1-48f4-b9c8-4fa84beb4592
local.conference.dateSEP 12-17, 1999
local.conference.locationPRAGUE, CZECH REPUBLIC
local.conference.title23rd International Epilepsy Congress
local.import.packageSS15
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage302
oaire.citation.issue4
oaire.citation.startPage297
oaire.citation.titleNEUROLOGICAL SCIENCES
oaire.citation.volume22
relation.isAuthorOfPublicationc359dea3-046f-4397-90d5-62e4bfc31869
relation.isAuthorOfPublication.latestForDiscoveryc359dea3-046f-4397-90d5-62e4bfc31869

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