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Polycaprolactone/Gelatin/Hyaluronic Acid Electrospun Scaffolds to Mimic Glioblastoma Extracellular Matrix

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dc.contributor.authorYILMAZ, BETÜL
dc.contributor.authorsUnal, Semra; Arslan, Sema; Yilmaz, Betul Karademir; Oktar, Faik Nuzhet; Ficai, Denisa; Ficai, Anton; Gunduz, Oguzhan
dc.date.accessioned2022-03-14T09:21:04Z
dc.date.available2022-03-14T09:21:04Z
dc.date.issued2020-06-11
dc.description.abstractGlioblastoma (GBM), one of the most malignant types of human brain tumor, is resistant to conventional treatments and is associated with poor survival. Since the 3D extracellular matrix (ECM) of GBM microenvironment plays a significant role on the tumor behavior, the engineering of the ECM will help us to get more information on the tumor behavior and to define novel therapeutic strategies. In this study, polycaprolactone (PCL)/gelatin(Gel)/hyaluronic acid(HA) composite scaffolds with aligned and randomly oriented nanofibers were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor. We investigated the effect of nanotopography and components of fibers on the mechanical, morphological, and hydrophilic properties of electrospun nanofiber as well as their biocompatibility properties. Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) have been used to investigate possible interactions between components. The mean fiber diameter in the nanofiber matrix was increased with the presence of HA at low collector rotation speed. Moreover, the rotational velocity of the collector affected the fiber diameters as well as their homogenous distribution. Water contact angle measurements confirmed that hyaluronic acid-incorporated aligned nanofibers were more hydrophilic than that of random nanofibers. In addition, PCL/Gel/HA nanofibrous scaffold (7.9 MPa) exhibited a significant decrease in tensile strength compared to PCL/Gel nanofibrous mat (19.2 MPa). In-vitro biocompatibilities of nanofiber scaffolds were tested with glioblastoma cells (U251), and the PCL/Gel/HA scaffolds with random nanofiber showed improved cell adhesion and proliferation. On the other hand, PCL/Gel/HA scaffolds with aligned nanofiber were found suitable for enhancing axon growth and elongation supporting intracellular communication. Based on these results, PCL/Gel/HA composite scaffolds are excellent candidates as a biomimetic matrix for GBM and the study of the tumor.
dc.identifier.doi10.3390/ma13112661
dc.identifier.eissn1996-1944
dc.identifier.pubmed32545241
dc.identifier.urihttps://hdl.handle.net/11424/243005
dc.identifier.wosWOS:000551495800237
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofMATERIALS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject3D extracellular matrix
dc.subjectglioblastoma tumor model
dc.subjectpolycaprolactone
dc.subjectgelatin
dc.subjecthyaluronic acid
dc.subjectnanofiber
dc.subjectIN-VITRO EVALUATION
dc.subjectHYALURONIC-ACID
dc.subjectFIBROUS SCAFFOLDS
dc.subjectGRAPHENE OXIDE
dc.subjectNANOFIBROUS SCAFFOLDS
dc.subjectMECHANICAL-PROPERTIES
dc.subjectCOMPOSITE SCAFFOLD
dc.subjectGELATIN
dc.subjectFABRICATION
dc.subjectCHITOSAN
dc.titlePolycaprolactone/Gelatin/Hyaluronic Acid Electrospun Scaffolds to Mimic Glioblastoma Extracellular Matrix
dc.typearticle
dspace.entity.typePublication
local.avesis.idf1a735f9-914e-4f0d-9818-bf623fe95147
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumber2661
local.journal.numberofpages16
local.journal.quartileQ1
oaire.citation.issue11
oaire.citation.titleMATERIALS
oaire.citation.volume13
relation.isAuthorOfPublication81633a07-e5fb-4760-b3ef-bf0878d87827
relation.isAuthorOfPublication.latestForDiscovery81633a07-e5fb-4760-b3ef-bf0878d87827

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