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Targeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways

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dc.contributor.authorYÜKSEL, MERAL
dc.contributor.authorsSeker U., Kavak D. E. , Guzel B. C. , Baygeldi S. B. , YÜKSEL M., Unay Demirel O., Irtegun Kandemir S., Sener D.
dc.date.accessioned2022-12-26T13:18:11Z
dc.date.available2022-12-26T13:18:11Z
dc.date.issued2022-12-01
dc.description.abstract© 2022 Wiley-VCH GmbH.Testicular ischaemia reperfusion (I/R) injury results with serious dysfunctions in testis. This study aims to explore effects of soluble guanylate cyclase (sGC) stimulator Riociguat on experimental testicular I/R injury in rats. Twenty-one male rats were divided into three groups (Control, IR and IRR). The control group was not exposed to any application. Bilateral testis from IR and IRR animals were rotated 720° in opposite directions for 3 h to induce experimental testicular ischaemia. Animals in IR and IRR groups were subjected to 3 h of reperfusion. Isotonic and Riociguat were administered to the animals 30 min prior reperfusion by oral gavage. At the end of experiment, animals were sacrificed and tissue samples were used for analyses. Riociguat treatment significantly decreased tissue malondialdehyde and Luminol levels compared to the IR group (p < 0.05). The pathological changes, pro-apoptotic proteins (Bax, Caspase 3, and Caspase 9) and apoptotic index in the IR group were down regulated in Riociguat treated animals (p < 0.05). Riociguat treatment was also significantly increased anti-apoptotic Bcl-2 expression, but alleviated tissue injury via modulating pro-inflammatory cytokine IL-1β levels and significantly (p < 0.05) down-regulating NF-κB activity. Moreover, mTOR and ERK phosphorylation increased in IR group (p < 0.05), but Riociguat treatment reduced protein phosphorylation. Our experiment indicated that targeting sGC might support surgical interventions in testicular I/R injury by modulating oxidative stress, inflammation, and apoptotic protein expression levels, but more detailed studies are required to explore the protective activity of Riociguat and underlying mechanisms in testicular I/R injury.
dc.identifier.citationSeker U., Kavak D. E. , Guzel B. C. , Baygeldi S. B. , YÜKSEL M., Unay Demirel O., Irtegun Kandemir S., Sener D., "Targeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways", Andrologia, cilt.54, sa.11, 2022
dc.identifier.doi10.1111/and.14616
dc.identifier.issn0303-4569
dc.identifier.issue11
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85140399446&origin=inward
dc.identifier.urihttps://hdl.handle.net/11424/284067
dc.identifier.volume54
dc.language.isoeng
dc.relation.ispartofAndrologia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectDahili Tıp Bilimleri
dc.subjectİç Hastalıkları
dc.subjectEndokrinoloji ve Metabolizma Hastalıkları
dc.subjectNefroloji
dc.subjectSağlık Bilimleri
dc.subjectMedicine
dc.subjectInternal Medicine Sciences
dc.subjectInternal Diseases
dc.subjectEndocrinology and Metabolic Diseases
dc.subjectNephrology
dc.subjectHealth Sciences
dc.subjectKlinik Tıp (MED)
dc.subjectKlinik Tıp
dc.subjectÜROLOJİ VE NEFROLOJİ
dc.subjectENDOKRİNOLOJİ VE METABOLİZMA
dc.subjectClinical Medicine (MED)
dc.subjectCLINICAL MEDICINE
dc.subjectUROLOGY & NEPHROLOGY
dc.subjectENDOCRINOLOGY & METABOLISM
dc.subjectEndokrinoloji
dc.subjectYaşam Bilimleri
dc.subjectÜroloji
dc.subjectEndocrinology
dc.subjectLife Sciences
dc.subjectUrology
dc.subjectischaemia reperfusion injury
dc.subjectrat
dc.subjectRiociguat
dc.subjectsGC stimulator
dc.subjecttestis
dc.subjectTORSION/DETORSION-INDUCED ISCHEMIA/REPERFUSION
dc.subjectOXIDATIVE STRESS
dc.subjectTESTIS ISCHEMIA
dc.subjectDAMAGE
dc.subjectAPOPTOSIS
dc.subjectRAPAMYCIN
dc.subjectDURATION
dc.subjectSYMPTOMS
dc.subjectPROTECTS
dc.subjectSALVAGE
dc.titleTargeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways
dc.typearticle
dspace.entity.typePublication
local.avesis.id6f1a7e18-72a6-469f-9038-1e1b6c3addf1
local.indexed.atWOS
local.indexed.atPUBMED
local.indexed.atSCOPUS
relation.isAuthorOfPublication8b13d479-2f3b-4180-bc71-7ad5a5625f1b
relation.isAuthorOfPublication.latestForDiscovery8b13d479-2f3b-4180-bc71-7ad5a5625f1b

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