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Resveratrol improves ifosfamide-induced Fanconi syndrome in rats

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dc.contributor.authorVELİOĞLU ÖĞÜNÇ, AYLİZ
dc.contributor.authorsSehirli, Ozer; Sakarcan, Abdullah; Velioglu-Oeguenc, Ayliz; Cetinel, Sule; Gedik, Nursal; Yegen, Berrak C.; Sener, Goeksel
dc.date.accessioned2022-03-12T17:33:06Z
dc.date.available2022-03-12T17:33:06Z
dc.date.issued2007
dc.description.abstractRegarding the mechanisms of ifosfamide (IFO)-induced urinary toxicity, several hypotheses have been put forward, among which oxidative stress and depletion of glutathione are suggested. This investigation elucidates the role of free radicals in IFO-induced toxicity and the protection by resveratrol, a natural phytoalexin. Wistar albino rats were injected intraperioneally with saline (0.9% NaCl; control), saline+ resveratrol (RVT; 10 mg/kg/day), ifosfamide (IFO; 50 mg/kg/day) or IFO+RVT for 5 days. Urine was collected for 24 h during the 5th day, and at the 120th h after the first injections, annuals were killed by decapitation and trunk blood was collected. Lactate dehydrogenase (LDH) activity, total antioxidant capacity (AOC) and pro-inflammatory cytokines TNF-alpha, IL-beta and IL-6 were assayed in plasma samples. Kidney and bladder tissues were obtained for biochemical and histological analysis. Formation of reactive oxygen species in the tissue samples was monitored by using chemiluminescence (CL) technique with luminol and lucigenin probes. The results demonstrated that IFO induced a Fanconi syndrome characterized by increased urinary sodium, phosphate, glucose and protein, along with increased serum creatinine and urea levels. On the other hand, RVT markedly ameliorated the severity of renal dysfunction induced by IFO. Furthermore IFO caused a significant decrease in plasma AOC. which was accompanied with significant increases in the levels of the pro-inflammatory mediators and LDH activity, while RVT treatment reversed all these biochemical indices. In the saline-treated IFO group, glutathione levels were decreased significantly, while the malondialdehyde levels, myeloperoxidase activity and collagen content were increased in both tissues, which were in parallel with the increases in CL values. In the RVT-treated IFO group, all of these oxidant responses were prevented significantly. Our results suggest that IFO causes oxidative damage in the renal and bladder tissues and resveratrol, via its antioxidant effects, protects these tissues. Therefore, its therapeutic role in proventing the development of chemotherapeutic drug-induced major toxicity in the urinary system requires further elucidation. (c) 2007 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.taap.2007.03.025
dc.identifier.eissn1096-0333
dc.identifier.issn0041-008X
dc.identifier.pubmed17481685
dc.identifier.urihttps://hdl.handle.net/11424/228769
dc.identifier.wosWOS:000247853900004
dc.language.isoeng
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.ispartofTOXICOLOGY AND APPLIED PHARMACOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectifosfamide
dc.subjectresveratrol
dc.subjectglutathione
dc.subjectlipid peroxidation
dc.subjectcytokine
dc.subjectmyeloperoxidase
dc.subjectWINE CONSTITUENT POLYPHENOL
dc.subjectRED WINE
dc.subjectMETABOLITE CHLOROACETALDEHYDE
dc.subjectOXIDATIVE STRESS
dc.subjectISCHEMIA/REPERFUSION
dc.subjectEXPRESSION
dc.subjectMECHANISM
dc.subjectTOXICITY
dc.subjectCELLS
dc.subjectSERUM
dc.titleResveratrol improves ifosfamide-induced Fanconi syndrome in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id24f20448-1925-486e-af4a-262d526ebd32
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages9
oaire.citation.endPage41
oaire.citation.issue1
oaire.citation.startPage33
oaire.citation.titleTOXICOLOGY AND APPLIED PHARMACOLOGY
oaire.citation.volume222
relation.isAuthorOfPublication13300bf6-ba96-4f87-9868-b0d2c86f572a
relation.isAuthorOfPublication.latestForDiscovery13300bf6-ba96-4f87-9868-b0d2c86f572a

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