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Comparison of telomere length and insulin-like growth factor-binding protein 7 promoter methylation between breast cancer tissues and adjacent normal tissues in Turkish women

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dc.contributor.authorGÜLLÜ AMURAN, GÖKÇE
dc.contributor.authorsKaya, Zehra; Akkiprik, Mustafa; Karabulut, Sevgi; Peker, Irem; Amuran, Gokce Gullu; Ozmen, Tolga; Gulluoglu, Bahadir M.; Kaya, Handan; Ozer, Ayse
dc.date.accessioned2022-03-14T08:29:29Z
dc.date.available2022-03-14T08:29:29Z
dc.date.issued2017-09
dc.description.abstractBackgroundBoth insulin-like growth factor-binding protein 7 (IGFBP7) and telomere length (TL) are associated with proliferation and senescence of human breast cancer. This study assessed the clinical significance of both TL and IGFBP7 methylation status in breast cancer tissues compared with adjacent normal tissues. We also investigated whether IGFBP7 methylation status could be affecting TL. MethodsTelomere length was measured by quantitative PCR to compare tumors with their adjacent normal tissues. The IGFBP7 promoter methylation status was evaluated by methylation-specific PCR and its expression levels were determined by western blotting. ResultsTelomeres were shorter in tumor tissues compared to controls (P<.0001). The mean TL was higher in breast cancer with invasive ductal carcinoma (IDC; n=72; P=.014) compared with other histological type (n=29), and TL in IDC with HER2 negative (n=53; P=.017) was higher than TL in IDC with HER2 positive (n=19). However, telomeres were shortened in advanced stages and growing tumors. IGFBP7 methylation was observed in 90% of tumor tissues and 59% of controls (P=.0002). Its frequency was significantly higher in IDC compared with invasive mixed carcinoma (IMC; P=.002) and it was not correlated either with protein expression or the other clinicopathological parameters. ConclusionThese results suggest that IGFBP7 promoter methylation and shorter TL in tumor compared with adjacent tissues may be predictive biomarkers for breast cancer. Telomere maintenance may be indicative of IDC and IDC with HER2 (-) of breast cancer. Further studies with larger number of cases are necessary to verify this association.
dc.identifier.doi10.1002/jcla.22083
dc.identifier.eissn1098-2825
dc.identifier.issn0887-8013
dc.identifier.pubmed27775181
dc.identifier.urihttps://hdl.handle.net/11424/241871
dc.identifier.wosWOS:000411716300014
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofJOURNAL OF CLINICAL LABORATORY ANALYSIS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectbreast cancer
dc.subjectinsulin-like growth factor-binding protein 7
dc.subjectmethylation
dc.subjecttelomere
dc.subjecttelomere length
dc.subjectTIME QUANTITATIVE PCR
dc.subjectMALIGNANT-TRANSFORMATION
dc.subjectCELLULAR SENESCENCE
dc.subjectCOLORECTAL-CANCER
dc.subjectGENE-EXPRESSION
dc.subjectDOWN-REGULATION
dc.subjectCELLS
dc.subjectIGFBP7
dc.subjectASSOCIATION
dc.subjectCARCINOMA
dc.titleComparison of telomere length and insulin-like growth factor-binding protein 7 promoter methylation between breast cancer tissues and adjacent normal tissues in Turkish women
dc.typearticle
dspace.entity.typePublication
local.avesis.idc995c173-ab94-4051-b9f7-b58a924a9580
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumbere22083
local.journal.numberofpages10
local.journal.quartileQ3
oaire.citation.issue5
oaire.citation.titleJOURNAL OF CLINICAL LABORATORY ANALYSIS
oaire.citation.volume31
relation.isAuthorOfPublication6b40e64d-7dc3-4fdc-9d2d-7cf7f2f5c14c
relation.isAuthorOfPublication.latestForDiscovery6b40e64d-7dc3-4fdc-9d2d-7cf7f2f5c14c

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