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In Vitro PDGF-B Gene Silencing Studies and In Vivo Delivery of siRNA to the Rat Kidney Using Chitosan/siRNA Nanoplexes

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dc.contributor.authorÖZBAŞ, SUNA
dc.contributor.authorsSalva, Emine; Ozbas Turan, Suna; Alan, Saadet; Akbuga, Julide
dc.date.accessioned2022-03-14T08:14:52Z
dc.date.available2022-03-14T08:14:52Z
dc.date.issued2016-05-12
dc.description.abstractThe targeting of specific genes responsible from onset and progression of kidney diseases offer a new therapeutic strategy in the field of renal gene therapy. The altered expression of platelet derived growth factor (PDGF) is an important marker of renal diseases. In this study, we investigated in vitro gene silencing efficiency of chitosan nanoplexes containing PDGF-B and PDGFR-beta targeted siRNAs in the kidney cell lines including HEK-293 and MDCK and delivery to the kidney as an in vivo delivery system. As a result, PDGF-B expression was significantly inhibited by co-delivery of chitosan/siPDGF-B+siPDGFR-beta nanoplexes prepared using in the different weight ratios (10/1, 20/1 and 50/1). When 20/1 and 50/1 weight ratios of chitosan nanoplexes were i.v. injected to rats, chitosan/FITC-siPDGFB nanoplexes were reached to kidney tissue at 4 h after intravenous injection. These results suggest that delivery of siRNA using chitosan nanoplexes may be effective for the therapy of kidney diseases.
dc.identifier.doi10.12991/mpj.20162082721
dc.identifier.issn1309-0801
dc.identifier.urihttps://hdl.handle.net/11424/241284
dc.identifier.wosWOS:000391173600005
dc.language.isoeng
dc.publisherMARMARA UNIV, FAC MEDICINE
dc.relation.ispartofMARMARA PHARMACEUTICAL JOURNAL
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectsiRNA
dc.subjectPDGF-B
dc.subjectPDGFR-beta
dc.subjectchitosan
dc.subjectnanoplexes
dc.subjectkidney
dc.subjectSMALL INTERFERING RNA
dc.subjectGROWTH-FACTOR
dc.subjectNANOPARTICLES
dc.subjectPOTENTIALS
dc.subjectTHERAPY
dc.subjectDISEASE
dc.subjectVEGF
dc.titleIn Vitro PDGF-B Gene Silencing Studies and In Vivo Delivery of siRNA to the Rat Kidney Using Chitosan/siRNA Nanoplexes
dc.typearticle
dspace.entity.typePublication
local.avesis.id4fc32067-fc6d-4a68-8a4a-757f68483536
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages6
oaire.citation.endPage268
oaire.citation.issue3
oaire.citation.startPage263
oaire.citation.titleMARMARA PHARMACEUTICAL JOURNAL
oaire.citation.volume20
relation.isAuthorOfPublicationd56d5fb1-37ec-4be6-8156-b58d67614019
relation.isAuthorOfPublication.latestForDiscoveryd56d5fb1-37ec-4be6-8156-b58d67614019

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